BMRB Entry 25281
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PDB ID: 2n2m
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR25281
MolProbity Validation Chart
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry
Citation: Marassi, Francesca; Ding, Yi; Schwieters, C.; Tian, Y.; Yao, Yong. "Backbone structure of Yersinia pestis Ail determined in micelles by NMR-restrained simulated annealing with implicit membrane solvation" J. Biomol. NMR ., .-. (2015).
Assembly members:
ATTACHMENT_INVASION_LOCUS_PROTEIN, polymer, 156 residues, 17492.549 Da.
Natural source: Common Name: enterobacteria Taxonomy ID: 187410 Superkingdom: Bacteria Kingdom: not available Genus/species: Yersinia pestis
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET-30b
Entity Sequences (FASTA):
ATTACHMENT_INVASION_LOCUS_PROTEIN: EGESSISIGYAQSRVKEDGY
KLDKNPRGFNLKYRYEFNND
WGVIGSFAQTRRGFEESVDG
FKLIDGDFKYYSVTAGPVFR
INEYVSLYGLLGAGHGKAKF
SSIFGQSESRSKTSLAYGAG
LQFNPHPNFVIDASYEYSKL
DDVKVGTWMLGAGYRF
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 280 |
| 15N chemical shifts | 146 |
| 1H chemical shifts | 146 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | entity | 1 |
Entities:
Entity 1, entity 156 residues - 17492.549 Da.
Residues 1-156 represent the mature sequence of the membrane protein after cleavage of the N-terminal signal sequence
| 1 | GLU | GLY | GLU | SER | SER | ILE | SER | ILE | GLY | TYR | ||||
| 2 | ALA | GLN | SER | ARG | VAL | LYS | GLU | ASP | GLY | TYR | ||||
| 3 | LYS | LEU | ASP | LYS | ASN | PRO | ARG | GLY | PHE | ASN | ||||
| 4 | LEU | LYS | TYR | ARG | TYR | GLU | PHE | ASN | ASN | ASP | ||||
| 5 | TRP | GLY | VAL | ILE | GLY | SER | PHE | ALA | GLN | THR | ||||
| 6 | ARG | ARG | GLY | PHE | GLU | GLU | SER | VAL | ASP | GLY | ||||
| 7 | PHE | LYS | LEU | ILE | ASP | GLY | ASP | PHE | LYS | TYR | ||||
| 8 | TYR | SER | VAL | THR | ALA | GLY | PRO | VAL | PHE | ARG | ||||
| 9 | ILE | ASN | GLU | TYR | VAL | SER | LEU | TYR | GLY | LEU | ||||
| 10 | LEU | GLY | ALA | GLY | HIS | GLY | LYS | ALA | LYS | PHE | ||||
| 11 | SER | SER | ILE | PHE | GLY | GLN | SER | GLU | SER | ARG | ||||
| 12 | SER | LYS | THR | SER | LEU | ALA | TYR | GLY | ALA | GLY | ||||
| 13 | LEU | GLN | PHE | ASN | PRO | HIS | PRO | ASN | PHE | VAL | ||||
| 14 | ILE | ASP | ALA | SER | TYR | GLU | TYR | SER | LYS | LEU | ||||
| 15 | ASP | ASP | VAL | LYS | VAL | GLY | THR | TRP | MET | LEU | ||||
| 16 | GLY | ALA | GLY | TYR | ARG | PHE |
Related Database Links:
| NCBI | NP_668646 |
| UNP | Q8D0Z7 |
| PDB | 2N2L 2N2M 3QRA 3QRC |
| DBJ | GAE10604 |
| EMBL | CAH22105 CAL21516 CCC18813 CFQ79902 CFU96389 |
| GB | AAB36601 AAM84897 AAS62746 ABG14310 ABG17563 |
| REF | WP_002222198 WP_002227864 WP_012104793 WP_012229027 WP_012303842 |
| SP | Q56957 |
| AlphaFold | Q8D0Z7 Q56957 |
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated peaks
SPARKY: Backbone
or all simulated peaks