BMRB Entry 25307

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PDB ID: 2mw8
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, SPARTA
BMRB Entry DOI: doi:10.13018/BMR25307
MolProbity Validation Chart

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Title:
solution structure of SATB1 homeodomain
Deposition date:
2014-10-31
Original release date:
2015-11-02
Authors:
Yamasaki, Kazuhiko; Yamasaki, Tomoko
Citation:

Citation: Yamasaki, Kazuhiko; Yamasaki, Tomoko. "The combination of sequence-specific and nonspecific DNA-binding modes of transcription factor SATB1"  Biochem. J. 473, 3321-3339 (2016).
PubMed: 27462121

Assembly members:

Assembly members:
SATB1, polymer, 71 residues, 7903.208 Da.

Natural source:

Natural source:   Common Name: human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET15b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
SATB1: GSHMNRQKTRPRTKISVEAL GILQSFIQDVGLYPDEEAIQ TLSAQLDLPKYTIIKFFQNQ RYYLKHHGKLK

Data sets:
Data typeCount
15N chemical shifts73
1H chemical shifts82

Additional metadata:

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Assembly:

Entity Assembly IDEntity NameEntity ID
1SATB1 homeodomain1

Entities:

Entity 1, SATB1 homeodomain 71 residues - 7903.208 Da.

1   GLYSERHISMETASNARGGLNLYSTHRARG
2   PROARGTHRLYSILESERVALGLUALALEU
3   GLYILELEUGLNSERPHEILEGLNASPVAL
4   GLYLEUTYRPROASPGLUGLUALAILEGLN
5   THRLEUSERALAGLNLEUASPLEUPROLYS
6   TYRTHRILEILELYSPHEPHEGLNASNGLN
7   ARGTYRTYRLEULYSHISHISGLYLYSLEU
8   LYS

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