BMRB Entry 25380

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PDB ID: 2mx0
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR25380
MolProbity Validation Chart

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Title:
Solution structure of HP0268 from Helicobacter pylori
Deposition date:
2014-12-05
Original release date:
2015-12-07
Authors:
Lee, Ki-Young
Citation:

Citation: Lee, Ki-Young; Lee, Kyu-Yeon; Kim, Ji-Hun; Lee, In-Gyun; Lee, Sung-Hee; Sim, Dae-Won; Won, Hyung-Sik; Lee, Bong-Jin. "Structure-based functional identification of Helicobacter pylori HP0268 as a nuclease with both DNA nicking and RNase activities"  Nucleic Acids Res. 43, 5194-5207 (2015).
PubMed: 25916841

Assembly members:

Assembly members:
HP0268, polymer, 82 residues, 9700.074 Da.

Natural source:

Natural source:   Common Name: e-proteobacteria   Taxonomy ID: 210   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Helicobacter pylori

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Helicobacter pylori   Vector: pET28a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
HP0268: SHMKLVLAKNTRKSDAKSVE LEDLYHEFSEDKRSIFYFAP TNAHKDMLKAVDFFKEKGHT AYLDEVRVSTDEKDFLYELH II

Data sets:
Data typeCount
13C chemical shifts328
15N chemical shifts80
1H chemical shifts459

Additional metadata:

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Assembly:

Entity Assembly IDEntity NameEntity ID
1entity1

Entities:

Entity 1, entity 82 residues - 9700.074 Da.

1   SERHISMETLYSLEUVALLEUALALYSASN
2   THRARGLYSSERASPALALYSSERVALGLU
3   LEUGLUASPLEUTYRHISGLUPHESERGLU
4   ASPLYSARGSERILEPHETYRPHEALAPRO
5   THRASNALAHISLYSASPMETLEULYSALA
6   VALASPPHEPHELYSGLULYSGLYHISTHR
7   ALATYRLEUASPGLUVALARGVALSERTHR
8   ASPGLULYSASPPHELEUTYRGLULEUHIS
9   ILEILE

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