BMRB Entry 25427

Name: DNA/RNA (28-MER)
Published: 2015-12-07

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PDB ID: 2mxs
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR25427
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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

DNA/RNA (28-MER)

Deposition date:

2015-01-14

Original release date:

2015-12-07

Authors:

Schmidtke, Sina; Duchardt-Ferner, Elke; Ohlenschlaeger, Oliver; Gottstein, Daniel; Wohnert, Jens

Citation:

Citation: Duchardt-Ferner, Elke; Schmidtke, Sina; Weigand, Julia; Ohlenschlaeger, Oliver; Wurm, Jan-Philip; Hammann, Christian; Suess, Beatrix; Wohnert, Jens. "What a difference an OH makes: conformational dynamics as the basis for ligand specificity of the neomycin sensing riboswitch" Angew. Chem. Int. Ed. Engl. 55, 1527-1530 (2016).
PubMed: 26661511

Assembly members:

Assembly members:
RNA_(27-MER), polymer, 27 residues, 3295.044 Da.
PAROMOMYCIN, non-polymer, 615.628 Da.

Natural source:

Natural source: Common Name: not available Taxonomy ID: not available Superkingdom: not available Kingdom: not available Genus/species: not available not available

Experimental source:

Experimental source: Production method: obtained from a collaborator Host organism: in vitro transcription

Entity Sequences (FASTA):

Entity Sequences (FASTA):
RNA_(27-MER): GGCUGCUUGUCCUUUAAUGG UCCAGUC

Data sets:

assigned_chemical_shifts

Data type	Count
13C chemical shifts	444
15N chemical shifts	20
1H chemical shifts	622

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	DNA/RNA (28-MER)	1
2	PAROMOMYCIN	2

Entities:

Entity 1, DNA/RNA (28-MER) 27 residues - 3295.044 Da.

1

G

C

U

G

C

U

G

U

2

C

U

A

U

G

3

U

C

A

G

U

C

Entity 2, PAROMOMYCIN - C23 H45 N5 O14 - 615.628 Da.

1

PAR

Name	Sample	Sample state	Sample conditions
2D 1H-1H NOESY	sample_1	isotropic	sample_conditions_1
2D 1H-15N HSQC	sample_2	isotropic	sample_conditions_1
2D 1H-13C HNCO	sample_2	isotropic	sample_conditions_1
2D 1H-15N H56C56C4N3H	sample_2	isotropic	sample_conditions_1
2D 1H-15N HSQC NH2 only	sample_2	isotropic	sample_conditions_1
2D 1H-13C HSQC aromatic	sample_3	isotropic	sample_conditions_2
2D 1H-13C HSQC aliphatic	sample_3	isotropic	sample_conditions_2
2D 1H-13C HSQC aliphatic	sample_4	isotropic	sample_conditions_2
3D 1H-13C NOESY-HSQC aliphatic	sample_3	isotropic	sample_conditions_2
3D 1H-13C NOESY-HMQC aromatic	sample_3	isotropic	sample_conditions_2
3D HCCH-COSY	sample_3	isotropic	sample_conditions_2
3D HCCH-TOCSY	sample_3	isotropic	sample_conditions_2
2D 1H-15N lrHSQC	sample_3	isotropic	sample_conditions_2
2D DQF-COSY	sample_3	isotropic	sample_conditions_2
2D 1H-31P HCP	sample_3	isotropic	sample_conditions_2
3D HCCH-TOCSY-E.COSY	sample_3	isotropic	sample_conditions_2
2D 13C filter NOESY	sample_3	isotropic	sample_conditions_2
2D 13C filter TOCSY	sample_3	isotropic	sample_conditions_2
2D quant 31P coupled 1H, 13C HSQC	sample_3	isotropic	sample_conditions_2
2D quant 31P coupled 1H-13C HMQC	sample_3	isotropic	sample_conditions_2

BMRB Entry 25427

Additional metadata:

Assembly:

Entities:

Samples:

Experiments:

Software:

NMR spectrometers: