BMRB Entry 25460

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PDB ID: 2myw
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR25460
MolProbity Validation Chart

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Title:
Solution structure of Fungus protein B9WZW9_MAGOR
Deposition date:
2015-01-30
Original release date:
2015-10-12
Authors:
de Guillen, Karine
Citation:

Citation: de Guillen, Karine; Ortiz-Vallejo, Diana; Gracy, Jerome; Fournier, Elisabeth; Kroj, Thomas; Padilla, Andre. "Structure analysis uncovers a highly diverse but structurally conserved effector family in phytopathogenic fungi"  PLOS PATHOG. 11, e1005228-e1005228 (2015).
PubMed: 26506000

Assembly members:

Assembly members:
AVR-Pia, polymer, 97 residues, 8885.165 Da.

Natural source:

Natural source:   Common Name: rice blast fungus   Taxonomy ID: 318829   Superkingdom: Eukaryota   Kingdom: Fungi   Genus/species: Magnaporthe oryzae

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET-SP

Entity Sequences (FASTA):

Entity Sequences (FASTA):
AVR-Pia: APQDNTSMGSSHHHHHHSSG RENLYFQGHMAAPARFCVYY DGHLPATRVLLMYVRIGTTA TITARGHEFEVEAKDQNCKV ILTNGKQAPDWLAAEPY

Data sets:
Data typeCount
13C chemical shifts228
15N chemical shifts81
1H chemical shifts510

Additional metadata:

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Assembly:

Entity Assembly IDEntity NameEntity ID
1AVR-Pia1

Entities:

Entity 1, AVR-Pia 97 residues - 8885.165 Da.

the sequence "APQDNTSMGSSHHHHHHSSGRENLYFQGHM" is the His-TAG and the TEV cleavage site

1   ALAPROGLNASPASNTHRSERMETGLYSER
2   SERHISHISHISHISHISHISSERSERGLY
3   ARGGLUASNLEUTYRPHEGLNGLYHISMET
4   ALAALAPROALAARGPHECYSVALTYRTYR
5   ASPGLYHISLEUPROALATHRARGVALLEU
6   LEUMETTYRVALARGILEGLYTHRTHRALA
7   THRILETHRALAARGGLYHISGLUPHEGLU
8   VALGLUALALYSASPGLNASNCYSLYSVAL
9   ILELEUTHRASNGLYLYSGLNALAPROASP
10   TRPLEUALAALAGLUPROTYR

Related Database Links:

UNP B9WZW9_MAGOR
AlphaFold B9WZW9

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks