BMRB Entry 25499

Name: NMR structure of the RRM3 domain of Hrb1
Published: 2015-11-30

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PDB ID: 2mzt
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR25499
MolProbity Validation Chart

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Nicholas Rego and David Koes
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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

NMR structure of the RRM3 domain of Hrb1

Deposition date:

2015-02-23

Original release date:

2015-11-30

Authors:

Martinez-Lumbreras, Santiago; Seraphin, Bertrand; Perez-Canadillas, Jose Manuel

Citation:

Citation: Martinez-Lumbreras, Santiago; Taverniti, Valerio; Zorrilla, Silvia; Seraphin, Bertrand; Perez-Canadillas, Jose Manuel. "Gbp2 interacts with THO/TREX through a novel type of RRM domain" Nucleic Acids Res. 44, 437-448 (2016).
PubMed: 26602689

Assembly members:

Assembly members:
RRM3, polymer, 100 residues, 11106.278 Da.

Natural source:

Natural source: Common Name: Baker's Yeast Taxonomy ID: 4932 Superkingdom: Eukaryota Kingdom: Fungi Genus/species: Saccharomyces cerevisiae

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET28

Entity Sequences (FASTA):

Entity Sequences (FASTA):
RRM3: GSVNEEARKFTENVVGGGER NRLIYCSNLPFSTAKSDLYD LFETIGKVNNAELRYDSKGA PTGIAVVEYDNVDDADVCIE RLNNYNYGGCDLDISYAKRL

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	288
15N chemical shifts	113
1H chemical shifts	665

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	RRM3	1

Entities:

Entity 1, RRM3 100 residues - 11106.278 Da.

From 357 to 454

1	GLY	SER	VAL	ASN	GLU	GLU	ALA	ARG	LYS	PHE
2	THR	GLU	ASN	VAL	VAL	GLY	GLY	GLY	GLU	ARG
3	ASN	ARG	LEU	ILE	TYR	CYS	SER	ASN	LEU	PRO
4	PHE	SER	THR	ALA	LYS	SER	ASP	LEU	TYR	ASP
5	LEU	PHE	GLU	THR	ILE	GLY	LYS	VAL	ASN	ASN
6	ALA	GLU	LEU	ARG	TYR	ASP	SER	LYS	GLY	ALA
7	PRO	THR	GLY	ILE	ALA	VAL	VAL	GLU	TYR	ASP
8	ASN	VAL	ASP	ASP	ALA	ASP	VAL	CYS	ILE	GLU
9	ARG	LEU	ASN	ASN	TYR	ASN	TYR	GLY	GLY	CYS
10	ASP	LEU	ASP	ILE	SER	TYR	ALA	LYS	ARG	LEU

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
2D 1H-13C HSQC	sample_2	isotropic	sample_conditions_1
2D 1H-1H TOCSY	sample_1	isotropic	sample_conditions_1
2D 1H-1H TOCSY	sample_2	isotropic	sample_conditions_1
2D 1H-1H NOESY	sample_1	isotropic	sample_conditions_1
2D 1H-1H NOESY	sample_2	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D HBHA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HCCH-TOCSY	sample_2	isotropic	sample_conditions_1

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks

BMRB Entry 25499

Additional metadata:

Assembly:

Entities:

Samples:

Experiments:

Software:

NMR spectrometers:

1	GLY	SER	VAL	ASN	GLU	GLU	ALA	ARG	LYS	PHE
2	THR	GLU	ASN	VAL	VAL	GLY	GLY	GLY	GLU	ARG
3	ASN	ARG	LEU	ILE	TYR	CYS	SER	ASN	LEU	PRO
4	PHE	SER	THR	ALA	LYS	SER	ASP	LEU	TYR	ASP
5	LEU	PHE	GLU	THR	ILE	GLY	LYS	VAL	ASN	ASN
6	ALA	GLU	LEU	ARG	TYR	ASP	SER	LYS	GLY	ALA
7	PRO	THR	GLY	ILE	ALA	VAL	VAL	GLU	TYR	ASP
8	ASN	VAL	ASP	ASP	ALA	ASP	VAL	CYS	ILE	GLU
9	ARG	LEU	ASN	ASN	TYR	ASN	TYR	GLY	GLY	CYS
10	ASP	LEU	ASP	ILE	SER	TYR	ALA	LYS	ARG	LEU

1	GLY	SER	VAL	ASN	GLU	GLU	ALA	ARG	LYS	PHE
2	THR	GLU	ASN	VAL	VAL	GLY	GLY	GLY	GLU	ARG
3	ASN	ARG	LEU	ILE	TYR	CYS	SER	ASN	LEU	PRO
4	PHE	SER	THR	ALA	LYS	SER	ASP	LEU	TYR	ASP
5	LEU	PHE	GLU	THR	ILE	GLY	LYS	VAL	ASN	ASN
6	ALA	GLU	LEU	ARG	TYR	ASP	SER	LYS	GLY	ALA
7	PRO	THR	GLY	ILE	ALA	VAL	VAL	GLU	TYR	ASP
8	ASN	VAL	ASP	ASP	ALA	ASP	VAL	CYS	ILE	GLU
9	ARG	LEU	ASN	ASN	TYR	ASN	TYR	GLY	GLY	CYS
10	ASP	LEU	ASP	ILE	SER	TYR	ALA	LYS	ARG	LEU

1	GLY	SER	VAL	ASN	GLU	GLU	ALA	ARG	LYS	PHE
2	THR	GLU	ASN	VAL	VAL	GLY	GLY	GLY	GLU	ARG
3	ASN	ARG	LEU	ILE	TYR	CYS	SER	ASN	LEU	PRO
4	PHE	SER	THR	ALA	LYS	SER	ASP	LEU	TYR	ASP
5	LEU	PHE	GLU	THR	ILE	GLY	LYS	VAL	ASN	ASN
6	ALA	GLU	LEU	ARG	TYR	ASP	SER	LYS	GLY	ALA
7	PRO	THR	GLY	ILE	ALA	VAL	VAL	GLU	TYR	ASP
8	ASN	VAL	ASP	ASP	ALA	ASP	VAL	CYS	ILE	GLU
9	ARG	LEU	ASN	ASN	TYR	ASN	TYR	GLY	GLY	CYS
10	ASP	LEU	ASP	ILE	SER	TYR	ALA	LYS	ARG	LEU