BMRB Entry 25534

Name: RNA structure determination by solid-state NMR spectroscopy
Published: 2015-05-08

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PDB ID: 2n0r
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR25534
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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

RNA structure determination by solid-state NMR spectroscopy

Deposition date:

2015-03-12

Original release date:

2015-05-08

Authors:

Marchanka, Alexander; Simon, Bernd; Althoff-Ospelt, Gerhard; Carlomagno, Teresa

Citation:

Citation: Marchanka, Alexander; Simon, Bernd; Althoff-Ospelt, Gerhard; Carlomagno, Teresa. "RNA structure determination by solid-state NMR spectroscopy" Nat. Commun. 6, 7024-7024 (2015).
PubMed: 25960310

Assembly members:

Assembly members:
26mer Box C/D RNA, polymer, 26 residues, 7450.573 Da.

Natural source:

Natural source: Common Name: E. coli Taxonomy ID: 562 Superkingdom: Bacteria Kingdom: not available Genus/species: Escherichia coli

Experimental source:

Experimental source: Production method: enzymatic semisynthesis

Entity Sequences (FASTA):

Entity Sequences (FASTA):
26mer Box C/D RNA: GCUGAGCUCGAAAGAGCAAU GAUGUC

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	173
15N chemical shifts	49
31P chemical shifts	9

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	entity	1

Entities:

Entity 1, entity 26 residues - 7450.573 Da.

1

G

C

U

G

A

G

C

U

C

G

2

A

G

A

G

C

A

U

3

G

A

U

G

U

C

Samples:

sample_1: entity, nucleotide-type-selective [U-99% 13C; U-99% 15N], 20 mg/mL; H2O 100%

sample_conditions_1: pH: 7.5; temperature: 260 K

Experiments:

Name	Sample	Sample state	Sample conditions
(13C,15N-TEDOR)-13C,13C PDSD	sample_1	solid	sample_conditions_1
13C-31P TEDOR	sample_1	solid	sample_conditions_1
CHHC	sample_1	solid	sample_conditions_1
NHHC	sample_1	solid	sample_conditions_1
CN-TEDOR	sample_1	solid	sample_conditions_1

Software:

CNS v1.2 - data analysis

NMR spectrometers:

Bruker Avance 700 MHz
Bruker Avance 600 MHz