BMRB Entry 25540

Click here to enlarge.

PDB ID: 2n0y
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, SPARTA
BMRB Entry DOI: doi:10.13018/BMR25540
MolProbity Validation Chart

NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry

Title:
NMR structure of the complex between the C-terminal domain of the Rift Valley fever virus protein NSs and the PH domain of the Tfb1 subunit of TFIIH.
Deposition date:
2015-03-18
Original release date:
2015-04-20
Authors:
Cyr, Normand; de la Fuente, Cynthia; Lecoq, Lauriane; Guendel, Irene; Chabot, Philippe; Kehn-Hall, Kylene; Omichinski, James
Citation:

Citation: Cyr, Normand; de la Fuente, Cynthia; Lecoq, Lauriane; Guendel, Irene; Chabot, Philippe; Kehn-Hall, Kylene; Omichinski, James. "A OmegaXaV motif in the Rift Valley fever virus NSs protein is essential for degrading p62, forming nuclear filaments and virulence."  Proc. Natl. Acad. Sci. U.S.A. 112, 6021-6026 (2015).
PubMed: 25918396

Assembly members:

Assembly members:
RNA_polymerase_II_transcription_factor_B_subunit_1, polymer, 115 residues, 12903.807 Da.
Non-structural_protein_NS-S, polymer, 24 residues, 2566.504 Da.

Natural source:

Natural source:   Common Name: baker's yeast   Taxonomy ID: 4932   Superkingdom: Eukarota   Kingdom: Fungi   Genus/species: Saccharomyces cerevisiae

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pGEX-2T

Entity Sequences (FASTA):

Entity Sequences (FASTA):
RNA_polymerase_II_transcription_factor_B_subunit_1: PSHSGAAIFEKVSGIIAINE DVSPAELTWRSTDGDKVHTV VLSTIDKLQATPASSEKMML RLIGKVDESKKRKDNEGNEV VPKPQRHMFSFNNRTVMDNI KMTLQQIISRYKDAD
Non-structural_protein_NS-S: GGGGYDVEMESEEESDDDGF VEVD

Data sets:
Data typeCount
13C chemical shifts597
15N chemical shifts143
1H chemical shifts953

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1RNA polymerase II transcription factor B subunit 11
2Non-structural protein NS-S2

Entities:

Entity 1, RNA polymerase II transcription factor B subunit 1 115 residues - 12903.807 Da.

1   PROSERHISSERGLYALAALAILEPHEGLU
2   LYSVALSERGLYILEILEALAILEASNGLU
3   ASPVALSERPROALAGLULEUTHRTRPARG
4   SERTHRASPGLYASPLYSVALHISTHRVAL
5   VALLEUSERTHRILEASPLYSLEUGLNALA
6   THRPROALASERSERGLULYSMETMETLEU
7   ARGLEUILEGLYLYSVALASPGLUSERLYS
8   LYSARGLYSASPASNGLUGLYASNGLUVAL
9   VALPROLYSPROGLNARGHISMETPHESER
10   PHEASNASNARGTHRVALMETASPASNILE
11   LYSMETTHRLEUGLNGLNILEILESERARG
12   TYRLYSASPALAASP

Entity 2, Non-structural protein NS-S 24 residues - 2566.504 Da.

Residues 242-246 were introduced to faciliate the purification and quantification of the protein.

1   GLYGLYGLYGLYTYRASPVALGLUMETGLU
2   SERGLUGLUGLUSERASPASPASPGLYPHE
3   VALGLUVALASP

Related Database Links:

SP P32776 P32776 P21698
REF NP_010597 NP_010597 YP_003848706
BMRB 6225 18229 18842 19791
PDB 1y5o 1Y5O 2GS0 2K2U 2L2I 2LOX 2M14 2MKR 2N0Y
DBJ GAA22531
EMBL CAY78811
GB AAA35143 AAB64747 AAU09707 AHY75284 AJP38011
TPG DAA12150
AlphaFold E9P948 P32776 A2SZX2

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks