Click here to enlarge.
PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, SPARTA
BMRB Entry DOI: doi:10.13018/BMR25568
MolProbity Validation Chart
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry
Citation: Lau, Carus; King, Glenn; Mobli, Mehdi. "Molecular basis of the interaction between gating modifier spider toxins and the voltage sensor of voltage-gated ion channels" Sci. Rep. 6, 34333-34333 (2016).
PubMed: 27677715
Assembly members:
entity, polymer, 35 residues, 4096.852 Da.
Natural source: Common Name: Spiders Taxonomy ID: 432528 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Grammostola rosea
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pLIC
Entity Sequences (FASTA):
entity: SECGKFMWKCKNSNDCCKDL
VCSSRWKWCVLASPF
Data type | Count |
13C chemical shifts | 154 |
15N chemical shifts | 39 |
1H chemical shifts | 240 |
Entity Assembly ID | Entity Name | Entity ID |
---|---|---|
1 | entity | 1 |
Entity 1, entity 35 residues - 4096.852 Da.
1 | SER | GLU | CYS | GLY | LYS | PHE | MET | TRP | LYS | CYS | ||||
2 | LYS | ASN | SER | ASN | ASP | CYS | CYS | LYS | ASP | LEU | ||||
3 | VAL | CYS | SER | SER | ARG | TRP | LYS | TRP | CYS | VAL | ||||
4 | LEU | ALA | SER | PRO | PHE |
sample_1: sodium acetate 20 mM; entity, [U-99% 13C; U-99% 15N], 1.7 mM; H2O 95%; D2O 5%
sample_conditions_1: ionic strength: 0 M; pH: 5; pressure: 1 atm; temperature: 298 K
Name | Sample | Sample state | Sample conditions |
---|---|---|---|
2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
3D HNCO | sample_1 | isotropic | sample_conditions_1 |
3D 1H-15N NOESY | sample_1 | isotropic | sample_conditions_1 |
2D 1H-13C HSQC aliphatic | sample_1 | isotropic | sample_conditions_1 |
2D 1H-13C HSQC aromatic | sample_1 | isotropic | sample_conditions_1 |
CYANA, Guntert, Mumenthaler and Wuthrich - structure solution
XEASY, Bartels et al. - peak picking
TALOS, Cornilescu, Delaglio and Bax - data analysis
TOPSPIN, Bruker Biospin - collection
Rowland_NMR_Toolkit v3, Jeffrey C. Hoch * Alan S. Stern - processing
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated peaks
SPARKY: Backbone
or all simulated peaks