BMRB Entry 25570
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR25570
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Title: Structure of the C-terminal membrane domain of HCV NS5B protein
Deposition date: 2015-04-15 Original release date: 2016-04-18
Authors: Montserret, Roland; Penin, Francois
Citation: Montserret, Roland; Penin, Francois. "Structure of the C-terminal membrane domain of HCV NS5B protein" J. Biol. Chem. ., .-..
Assembly members:
entity, polymer, 30 residues, 3483.139 Da.
Natural source: Common Name: not available Taxonomy ID: 31646 Superkingdom: Viruses Kingdom: not available Genus/species: Hepacivirus Hepatitis C virus
Experimental source: Production method: chemical synthesis
Entity Sequences (FASTA):
entity: HSVSHARPRWFWFSLLLLAA
GVGIYLLPNR
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 102 |
| 15N chemical shifts | 24 |
| 1H chemical shifts | 212 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | ns5bc | 1 |
Entities:
Entity 1, ns5bc 30 residues - 3483.139 Da.
NS5B genotype 1a
| 1 | HIS | SER | VAL | SER | HIS | ALA | ARG | PRO | ARG | TRP | |
| 2 | PHE | TRP | PHE | SER | LEU | LEU | LEU | LEU | ALA | ALA | |
| 3 | GLY | VAL | GLY | ILE | TYR | LEU | LEU | PRO | ASN | ARG |
Samples:
sample_1: entity 1.4 ± 0.05 mM; SDS, [U-2H], 1 M; H2O 95%; D2O, [U-2H], 5%
sample_conditions_1: pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-1H TOCSY | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-1H NOESY | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-13C HSQC | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-1H TOCSY | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-1H NOESY | sample_1 | isotropic | sample_conditions_1 |
Software:
TOPSPIN, Bruker Biospin - collection
SPARKY, Goddard - chemical shift assignment
TALOS, Cornilescu, Delaglio and Bax - geometry optimization
X-PLOR_NIH v2.34, Schwieters, Kuszewski, Tjandra and Clore - structure solution
NMR spectrometers:
- Bruker Avance 500 MHz
- Bruker Avance 1000 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts