BMRB Entry 25584
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PDB ID: 2n23
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR25584
MolProbity Validation Chart
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry
Citation: Morse, Thomas; Lecoq, Lauriane; Raiola, Luca; Arseneault, Genevieve; Omichinski, James. "Structural Characterization of the Complex between the N-terminal Transactivation Domain of EKLF and the p62/Tfb1 subunit of TFIIH" .
Assembly members:
RNA_polymerase_II_transcription_factor_B_subunit_1, polymer, 115 residues, 12903.807 Da.
N-terminal_transactivation_domain_of_Krueppel-like_factor_1, polymer, 19 residues, 2359.502 Da.
Natural source: Common Name: baker's yeast Taxonomy ID: 4932 Superkingdom: Eukaryota Kingdom: Fungi Genus/species: Saccharomyces cerevisiae
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pGEX-TEV
Entity Sequences (FASTA):
RNA_polymerase_II_transcription_factor_B_subunit_1: PSHSGAAIFEKVSGIIAINE
DVSPAELTWRSTDGDKVHTV
VLSTIDKLQATPASSEKMML
RLIGKVDESKKRKDNEGNEV
VPKPQRHMFSFNNRTVMDNI
KMTLQQIISRYKDAD
N-terminal_transactivation_domain_of_Krueppel-like_factor_1: DTQDDFLKWWRSEEAQDMG
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 578 |
| 15N chemical shifts | 144 |
| 1H chemical shifts | 938 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | entity_1 | 1 |
| 2 | entity_2 | 2 |
Entities:
Entity 1, entity_1 115 residues - 12903.807 Da.
| 1 | PRO | SER | HIS | SER | GLY | ALA | ALA | ILE | PHE | GLU | ||||
| 2 | LYS | VAL | SER | GLY | ILE | ILE | ALA | ILE | ASN | GLU | ||||
| 3 | ASP | VAL | SER | PRO | ALA | GLU | LEU | THR | TRP | ARG | ||||
| 4 | SER | THR | ASP | GLY | ASP | LYS | VAL | HIS | THR | VAL | ||||
| 5 | VAL | LEU | SER | THR | ILE | ASP | LYS | LEU | GLN | ALA | ||||
| 6 | THR | PRO | ALA | SER | SER | GLU | LYS | MET | MET | LEU | ||||
| 7 | ARG | LEU | ILE | GLY | LYS | VAL | ASP | GLU | SER | LYS | ||||
| 8 | LYS | ARG | LYS | ASP | ASN | GLU | GLY | ASN | GLU | VAL | ||||
| 9 | VAL | PRO | LYS | PRO | GLN | ARG | HIS | MET | PHE | SER | ||||
| 10 | PHE | ASN | ASN | ARG | THR | VAL | MET | ASP | ASN | ILE | ||||
| 11 | LYS | MET | THR | LEU | GLN | GLN | ILE | ILE | SER | ARG | ||||
| 12 | TYR | LYS | ASP | ALA | ASP |
Entity 2, entity_2 19 residues - 2359.502 Da.
| 1 | ASP | THR | GLN | ASP | ASP | PHE | LEU | LYS | TRP | TRP | ||||
| 2 | ARG | SER | GLU | GLU | ALA | GLN | ASP | MET | GLY |
Related Database Links:
| PDB | 1y5o |
| BMRB | 6225 |
| SP | P32776 Q13351 |
| REF | NP_010597 K09204 |
| NCBI | 10661 |
| AlphaFold | E9P948 Q92899 |
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated peaks
SPARKY: Backbone
or all simulated peaks