BMRB Entry 25604

Name: Solution structure of a double base-pair inversion mutant of murine tumour necrosis factor alpha CDE-23 RNA
Published: 2015-08-03

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PDB ID: 2n2p
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR25604
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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Solution structure of a double base-pair inversion mutant of murine tumour necrosis factor alpha CDE-23 RNA

Deposition date:

2015-05-11

Original release date:

2015-08-03

Authors:

Codutti, Luca; Leppek, Katrin; Zalesak, Jan; Windeisen, Volker; Masiewicz, Pawel; Stoecklin, Georg; Carlomagno, Teresa

Citation:

Citation: Codutti, Luca; Leppek, Katrin; Zalesak, Jan; Windeisen, Volker; Masiewicz, Pawel; Stoecklin, Georg; Carlomagno, Teresa. "A Distinct, Sequence-Induced Conformation Is Required for Recognition of the Constitutive Decay Element RNA by Roquin" Structure 23, 1437-1447 (2015).
PubMed: 26165594

Assembly members:

Assembly members:
RNA_(5'-R(P*GP*CP*AP*UP*GP*UP*UP*UP*AP*GP*UP*GP*UP*CP*UP*AP*AP*AP*CP*GP*GP*UP*U)-3'), polymer, 23 residues, 7343.398 Da.

Natural source:

Natural source: Common Name: not available Taxonomy ID: not available Superkingdom: not available Kingdom: not available Genus/species: not available not available

Experimental source:

Experimental source: Production method: enzymatic semisynthesis

Entity Sequences (FASTA):

Entity Sequences (FASTA):
RNA_(5'-R(P*GP*CP*AP*UP*GP*UP*UP*UP*AP*GP*UP*GP*UP*CP*UP*AP*AP*AP*CP*GP*GP*UP*U)-3'): GCAUGUUUAGUGUCUAAACG GUU

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	151
1H chemical shifts	180
31P chemical shifts	22

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	RNA (5'-R(PGPCPAPUPGPUPUPUPAPGPUPGPUPCPUPAPAPAPCPGPGPUP*U)-3')	1

Entities:

Entity 1, RNA (5'-R(P*GP*CP*AP*UP*GP*UP*UP*UP*AP*GP*UP*GP*UP*CP*UP*AP*AP*AP*CP*GP*GP*UP*U)-3') 23 residues - 7343.398 Da.

1

G

C

A

U

G

U

A

G

2

U

G

U

C

U

A

C

G

3

G

U

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
2D 1H-13C HSQC	sample_1	isotropic	sample_conditions_1
immino-1H-1H NOESY	sample_2	isotropic	sample_conditions_1
3D-HCCH-COSY-TOCSY	sample_1	isotropic	sample_conditions_1
3D HsCNb/HbCNb	sample_1	isotropic	sample_conditions_1
2D HNN-COSY	sample_2	isotropic	sample_conditions_1
3D 13C-edited NOESY	sample_1	isotropic	sample_conditions_1
2D COSY	sample_1	isotropic	sample_conditions_1
1H-31P HET-COR(P,H-COSY-H,C-HMQC)	sample_1	isotropic	sample_conditions_1
T1-13C-EDIT relaxation	sample_1	isotropic	sample_conditions_1
T1rho-13C-EDIT relaxation	sample_1	isotropic	sample_conditions_1
13C-base edited IPAP	sample_3	anisotropic	sample_conditions_1
13C-sugar edited IPAP	sample_3	anisotropic	sample_conditions_1

BMRB Entry 25604

Additional metadata:

Assembly:

Entities:

Samples:

Experiments:

Software:

NMR spectrometers: