BMRB Entry 25703

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PDB ID: 2n5b
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR25703
MolProbity Validation Chart

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Title:
Structure of the reduced and oxidized state of the mutant D24A of yeast thioredoxin 1: insight into the mechanism for the closing of the water cavity
Deposition date:
2015-07-13
Original release date:
2015-10-26
Authors:
Iqbal, Anwar; Moraes, Adolfo; Valente, Ana Paula; Almeida, Fabio
Citation:

Citation: Iqbal, Anwar; Moraes, Adolfo; Valente, Ana Paula; Almeida, Fabio. "Structures of the reduced and oxidized state of the mutant D24A of yeast thioredoxin 1: insights into the mechanism for the closing of the water cavity"  J. Biomol. NMR 63, 417-423 (2015).
PubMed: 26482062

Assembly members:

Assembly members:
Yeast_Thioredoxin_1_Oxidized, polymer, 103 residues, 11201.019 Da.

Natural source:

Natural source:   Common Name: baker's yeast   Taxonomy ID: not available   Superkingdom: Eukaryota   Kingdom: Fungi   Genus/species: SACCHAROMYCES CEREVISIAE

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: PET17B

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Yeast_Thioredoxin_1_Oxidized: MVTQFKTASEFDSAIAQDKL VVVAFYATWCGPCKMIAPMI EKFSEQYPQADFYKLDVDEL GDVAQKNEVSAMPTLLLFKN GKEVAKVVGANPAAIKQAIA ANA

Data sets:
Data typeCount
13C chemical shifts421
15N chemical shifts101
1H chemical shifts656

Additional metadata:

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Assembly:

Entity Assembly IDEntity NameEntity ID
1oxidized form of thioredoxin 1 from Sacharomyces cerevisiae1

Entities:

Entity 1, oxidized form of thioredoxin 1 from Sacharomyces cerevisiae 103 residues - 11201.019 Da.

1   METVALTHRGLNPHELYSTHRALASERGLU
2   PHEASPSERALAILEALAGLNASPLYSLEU
3   VALVALVALALAPHETYRALATHRTRPCYS
4   GLYPROCYSLYSMETILEALAPROMETILE
5   GLULYSPHESERGLUGLNTYRPROGLNALA
6   ASPPHETYRLYSLEUASPVALASPGLULEU
7   GLYASPVALALAGLNLYSASNGLUVALSER
8   ALAMETPROTHRLEULEULEUPHELYSASN
9   GLYLYSGLUVALALALYSVALVALGLYALA
10   ASNPROALAALAILELYSGLNALAILEALA
11   ALAASNALA

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