BMRB Entry 25776

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PDB ID: 2n6p
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR25776
MolProbity Validation Chart

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Title:
Solution NMR structure of Outer Membrane Protein G P92A mutant from Pseudomonas aeruginosa
Deposition date:
2015-08-27
Original release date:
2015-12-28
Authors:
Kucharska, Iga; Seelheim, Patrick; Edrington, Thomas; Liang, Binyong; Tamm, Lukas
Citation:

Citation: Kucharska, Iga; Seelheim, Patrick; Edrington, Thomas; Liang, Binyong; Tamm, Lukas. "OprG Harnesses the Dynamics of its Extracellular Loops to Transport Small Amino Acids across the Outer Membrane of Pseudomonas aeruginosa"  Structure 23, 2234-2245 (2015).
PubMed: 26655471

Assembly members:

Assembly members:
entity, polymer, 215 residues, 22998.037 Da.

Natural source:

Natural source:   Common Name: g-proteobacteria   Taxonomy ID: 287   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Pseudomonas aeruginosa

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: Pet30a+

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity: MHKAGDFIIRGGFATVDPDD SSSDIKLDGAKQRGTKATVD SDTQLGLTFTYMFADKWGVE LVAATPFNHQVDVKGLGPGL DGKLADIKQLPATLLLQYYP MGGTNSAFQPYGGLGVNYTT FFDEDLASNRKAQGFSSMKL QDSWGLAGELGFDYMLNEHA LFNMAVWYMDIDTKASINGP SALGVNKTKVDVDVDPWVYM IGFGYKFLEHHHHHH

Data sets:
Data typeCount
13C chemical shifts488
15N chemical shifts155
1H chemical shifts155

Additional metadata:

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  • Samples and Experiments
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Assembly:

Entity Assembly IDEntity NameEntity ID
1entity1

Entities:

Entity 1, entity 215 residues - 22998.037 Da.

1   METHISLYSALAGLYASPPHEILEILEARG
2   GLYGLYPHEALATHRVALASPPROASPASP
3   SERSERSERASPILELYSLEUASPGLYALA
4   LYSGLNARGGLYTHRLYSALATHRVALASP
5   SERASPTHRGLNLEUGLYLEUTHRPHETHR
6   TYRMETPHEALAASPLYSTRPGLYVALGLU
7   LEUVALALAALATHRPROPHEASNHISGLN
8   VALASPVALLYSGLYLEUGLYPROGLYLEU
9   ASPGLYLYSLEUALAASPILELYSGLNLEU
10   PROALATHRLEULEULEUGLNTYRTYRPRO
11   METGLYGLYTHRASNSERALAPHEGLNPRO
12   TYRGLYGLYLEUGLYVALASNTYRTHRTHR
13   PHEPHEASPGLUASPLEUALASERASNARG
14   LYSALAGLNGLYPHESERSERMETLYSLEU
15   GLNASPSERTRPGLYLEUALAGLYGLULEU
16   GLYPHEASPTYRMETLEUASNGLUHISALA
17   LEUPHEASNMETALAVALTRPTYRMETASP
18   ILEASPTHRLYSALASERILEASNGLYPRO
19   SERALALEUGLYVALASNLYSTHRLYSVAL
20   ASPVALASPVALASPPROTRPVALTYRMET
21   ILEGLYPHEGLYTYRLYSPHELEUGLUHIS
22   HISHISHISHISHIS

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