BMRB Entry 25796
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PDB ID: 2n77
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: LACS
BMRB Entry DOI: doi:10.13018/BMR25796
MolProbity Validation Chart
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry
Citation: Wang, Xu; Putkey, John. "PEP-19 Modulates Calcium Binding to Calmodulin by Electrostatic Steering" Nat. Commun. 7, 13583-13583 (2016).
PubMed: 27876793
Assembly members:
C-CaM, polymer, 73 residues, 8416.260 Da.
PEP-19, polymer, 62 residues, 6775.41 Da.
Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET23d
Entity Sequences (FASTA):
C-CaM: MKDTDSEEEIREAFRVFDKD
GNGYISAAELRHVMTNLGEK
LTDEEVDEMIREADIDGDGQ
VNYEEFVQMMTAK
PEP-19: MAERQGAGATNGKDKTSGEN
DGQKKVQEEFDIDMDAPETE
RAAVAIQSQFRKFQKKKAGS
QS
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 430 |
| 15N chemical shifts | 112 |
| 1H chemical shifts | 716 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | entity_1 | 1 |
| 2 | entity_2 | 2 |
Entities:
Entity 1, entity_1 73 residues - 8416.260 Da.
Isolated C-domain of calmodulin from residue M76 to K148
| 1 | MET | LYS | ASP | THR | ASP | SER | GLU | GLU | GLU | ILE | ||||
| 2 | ARG | GLU | ALA | PHE | ARG | VAL | PHE | ASP | LYS | ASP | ||||
| 3 | GLY | ASN | GLY | TYR | ILE | SER | ALA | ALA | GLU | LEU | ||||
| 4 | ARG | HIS | VAL | MET | THR | ASN | LEU | GLY | GLU | LYS | ||||
| 5 | LEU | THR | ASP | GLU | GLU | VAL | ASP | GLU | MET | ILE | ||||
| 6 | ARG | GLU | ALA | ASP | ILE | ASP | GLY | ASP | GLY | GLN | ||||
| 7 | VAL | ASN | TYR | GLU | GLU | PHE | VAL | GLN | MET | MET | ||||
| 8 | THR | ALA | LYS |
Entity 2, entity_2 62 residues - 6775.41 Da.
| 1 | MET | ALA | GLU | ARG | GLN | GLY | ALA | GLY | ALA | THR | ||||
| 2 | ASN | GLY | LYS | ASP | LYS | THR | SER | GLY | GLU | ASN | ||||
| 3 | ASP | GLY | GLN | LYS | LYS | VAL | GLN | GLU | GLU | PHE | ||||
| 4 | ASP | ILE | ASP | MET | ASP | ALA | PRO | GLU | THR | GLU | ||||
| 5 | ARG | ALA | ALA | VAL | ALA | ILE | GLN | SER | GLN | PHE | ||||
| 6 | ARG | LYS | PHE | GLN | LYS | LYS | LYS | ALA | GLY | SER | ||||
| 7 | GLN | SER |
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