BMRB Entry 25800

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PDB ID: 2n7c
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR25800
MolProbity Validation Chart

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Title:
Solution structure of Plasmodium falciparum SR1-RRM1 in complex with ACAUCA RNA
Deposition date:
2015-09-08
Original release date:
2016-10-06
Authors:
Ganguly, Akshay; Verma, Garima; Bhavesh, Neel Sarovar
Citation:

Citation: Ganguly, Akshay; Verma, Garima; Bhavesh, Neel Sarovar. "The N-terminal RNA Recognition Motif of PfSR1 Confers Semi-specificity for Pyrimidines during RNA Recognition"  J. Mol. Biol. ., .-. (2018).
PubMed: 30500338

Assembly members:

Assembly members:
PfSR1-RRM1, polymer, 89 residues, 9997.284 Da.
RNA_(5'-R(*AP*CP*AP*UP*CP*A)-3'), polymer, 6 residues, 1859.203 Da.

Natural source:

Natural source:   Common Name: malaria parasite P. falciparum   Taxonomy ID: 5833   Superkingdom: Eukaryota   Kingdom: not available   Genus/species: Plasmodium falciparum

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pet28a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
PfSR1-RRM1: GSHMVIRESVSRIYVGNLPS HVSSRDVENEFRKYGNILKC DVKKTVSGAAFAFIEFEDAR DAADAIKEKDGCDFEGNKLR VEVPFNARE
RNA_(5'-R(*AP*CP*AP*UP*CP*A)-3'): ACAUCA

Data sets:
Data typeCount
13C chemical shifts398
15N chemical shifts84
1H chemical shifts374

Additional metadata:

  • Assembly
  • Samples and Experiments
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  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1entity_11
2RNA (5'-R(*AP*CP*AP*UP*CP*A)-3')2

Entities:

Entity 1, entity_1 89 residues - 9997.284 Da.

G-2, S-1, H0 are from pet28a vector

1   GLYSERHISMETVALILEARGGLUSERVAL
2   SERARGILETYRVALGLYASNLEUPROSER
3   HISVALSERSERARGASPVALGLUASNGLU
4   PHEARGLYSTYRGLYASNILELEULYSCYS
5   ASPVALLYSLYSTHRVALSERGLYALAALA
6   PHEALAPHEILEGLUPHEGLUASPALAARG
7   ASPALAALAASPALAILELYSGLULYSASP
8   GLYCYSASPPHEGLUGLYASNLYSLEUARG
9   VALGLUVALPROPHEASNALAARGGLU

Entity 2, RNA (5'-R(*AP*CP*AP*UP*CP*A)-3') 6 residues - 1859.203 Da.

1   ACAUCA

Related Database Links:

NCBI XP_001351730.2

Download HSQC peak lists in one of the following formats:
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SPARKY: Backbone or all simulated peaks