BMRB Entry 25802

Name: Resonance assignments of [15N, 13C] human DCL-1 (CD302) extracellular domain
Published: 2016-01-06

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PDB ID: 2nan
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR25802
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Nicholas Rego and David Koes
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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Resonance assignments of [15N, 13C] human DCL-1 (CD302) extracellular domain

Deposition date:

2015-09-09

Original release date:

2016-01-06

Authors:

Pospisilova, Eliska; Kavan, Daniel; Novak, Petr; Chmelik, Josef

Citation:

Citation: Pospisilova, Eliska; Kavan, Daniel; Novak, Petr; Chmelik, Josef. "1H, 13C and 15N resonance assignments of human DCL-1 (CD302) extracellular domain" Biomol. NMR Assign. 10, 189-192 (2016).
PubMed: 26725057

Assembly members:

Assembly members:
CD302, polymer, 140 residues, Formula weight is not available

Natural source:

Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET-30a(+)

Entity Sequences (FASTA):

Entity Sequences (FASTA):
CD302: MDCPSSTWIQFQDSCYIFLQ EAIKVESIEDVRNQCTDHGA DMISIHNEEENAFILDTLKK QWKGPDDILLGMFYDTDDAS FKWFDNSNMTFDKWTDQDDD EDLVDTCAFLHIKTGEWKKG NCEVSSVEGTLCKTAIPYKR

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	627
15N chemical shifts	151
1H chemical shifts	962

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	CD302	1

Entities:

Entity 1, CD302 140 residues - Formula weight is not available

Construct contains initial MET (MET22) which is not in full native sequence.

1	MET	ASP	CYS	PRO	SER	SER	THR	TRP	ILE	GLN
2	PHE	GLN	ASP	SER	CYS	TYR	ILE	PHE	LEU	GLN
3	GLU	ALA	ILE	LYS	VAL	GLU	SER	ILE	GLU	ASP
4	VAL	ARG	ASN	GLN	CYS	THR	ASP	HIS	GLY	ALA
5	ASP	MET	ILE	SER	ILE	HIS	ASN	GLU	GLU	GLU
6	ASN	ALA	PHE	ILE	LEU	ASP	THR	LEU	LYS	LYS
7	GLN	TRP	LYS	GLY	PRO	ASP	ASP	ILE	LEU	LEU
8	GLY	MET	PHE	TYR	ASP	THR	ASP	ASP	ALA	SER
9	PHE	LYS	TRP	PHE	ASP	ASN	SER	ASN	MET	THR
10	PHE	ASP	LYS	TRP	THR	ASP	GLN	ASP	ASP	ASP
11	GLU	ASP	LEU	VAL	ASP	THR	CYS	ALA	PHE	LEU
12	HIS	ILE	LYS	THR	GLY	GLU	TRP	LYS	LYS	GLY
13	ASN	CYS	GLU	VAL	SER	SER	VAL	GLU	GLY	THR
14	LEU	CYS	LYS	THR	ALA	ILE	PRO	TYR	LYS	ARG

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HN(CA)CO	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D HN(CO)CA	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
2D 1H-13C HSQC aliphatic	sample_1	isotropic	sample_conditions_1
2D 1H-13C HSQC aromatic	sample_1	isotropic	sample_conditions_1
3D 1H-15N NOESY	sample_1	isotropic	sample_conditions_1
3D 1H-13C NOESY aliphatic	sample_1	isotropic	sample_conditions_1
3D 1H-13C NOESY aromatic	sample_1	isotropic	sample_conditions_1
3D 1H-15N TOCSY	sample_1	isotropic	sample_conditions_1
3D H(CC)(CO)NH	sample_1	isotropic	sample_conditions_1
3D (H)CC(CO)NH	sample_1	isotropic	sample_conditions_1
2D (HB)CB(CGCD)HD	sample_1	isotropic	sample_conditions_1
3D HCCH-TOCSY	sample_1	isotropic	sample_conditions_1
3D HCCH-TOCSY aromatic	sample_1	isotropic	sample_conditions_1

UNP	Q8IX05
AlphaFold	Q15009

BMRB Entry 25802

Additional metadata:

Assembly:

Entities:

Samples:

Experiments:

Software:

NMR spectrometers:

Related Database Links:

1	MET	ASP	CYS	PRO	SER	SER	THR	TRP	ILE	GLN
2	PHE	GLN	ASP	SER	CYS	TYR	ILE	PHE	LEU	GLN
3	GLU	ALA	ILE	LYS	VAL	GLU	SER	ILE	GLU	ASP
4	VAL	ARG	ASN	GLN	CYS	THR	ASP	HIS	GLY	ALA
5	ASP	MET	ILE	SER	ILE	HIS	ASN	GLU	GLU	GLU
6	ASN	ALA	PHE	ILE	LEU	ASP	THR	LEU	LYS	LYS
7	GLN	TRP	LYS	GLY	PRO	ASP	ASP	ILE	LEU	LEU
8	GLY	MET	PHE	TYR	ASP	THR	ASP	ASP	ALA	SER
9	PHE	LYS	TRP	PHE	ASP	ASN	SER	ASN	MET	THR
10	PHE	ASP	LYS	TRP	THR	ASP	GLN	ASP	ASP	ASP
11	GLU	ASP	LEU	VAL	ASP	THR	CYS	ALA	PHE	LEU
12	HIS	ILE	LYS	THR	GLY	GLU	TRP	LYS	LYS	GLY
13	ASN	CYS	GLU	VAL	SER	SER	VAL	GLU	GLY	THR
14	LEU	CYS	LYS	THR	ALA	ILE	PRO	TYR	LYS	ARG

1	MET	ASP	CYS	PRO	SER	SER	THR	TRP	ILE	GLN
2	PHE	GLN	ASP	SER	CYS	TYR	ILE	PHE	LEU	GLN
3	GLU	ALA	ILE	LYS	VAL	GLU	SER	ILE	GLU	ASP
4	VAL	ARG	ASN	GLN	CYS	THR	ASP	HIS	GLY	ALA
5	ASP	MET	ILE	SER	ILE	HIS	ASN	GLU	GLU	GLU
6	ASN	ALA	PHE	ILE	LEU	ASP	THR	LEU	LYS	LYS
7	GLN	TRP	LYS	GLY	PRO	ASP	ASP	ILE	LEU	LEU
8	GLY	MET	PHE	TYR	ASP	THR	ASP	ASP	ALA	SER
9	PHE	LYS	TRP	PHE	ASP	ASN	SER	ASN	MET	THR
10	PHE	ASP	LYS	TRP	THR	ASP	GLN	ASP	ASP	ASP
11	GLU	ASP	LEU	VAL	ASP	THR	CYS	ALA	PHE	LEU
12	HIS	ILE	LYS	THR	GLY	GLU	TRP	LYS	LYS	GLY
13	ASN	CYS	GLU	VAL	SER	SER	VAL	GLU	GLY	THR
14	LEU	CYS	LYS	THR	ALA	ILE	PRO	TYR	LYS	ARG

1	MET	ASP	CYS	PRO	SER	SER	THR	TRP	ILE	GLN
2	PHE	GLN	ASP	SER	CYS	TYR	ILE	PHE	LEU	GLN
3	GLU	ALA	ILE	LYS	VAL	GLU	SER	ILE	GLU	ASP
4	VAL	ARG	ASN	GLN	CYS	THR	ASP	HIS	GLY	ALA
5	ASP	MET	ILE	SER	ILE	HIS	ASN	GLU	GLU	GLU
6	ASN	ALA	PHE	ILE	LEU	ASP	THR	LEU	LYS	LYS
7	GLN	TRP	LYS	GLY	PRO	ASP	ASP	ILE	LEU	LEU
8	GLY	MET	PHE	TYR	ASP	THR	ASP	ASP	ALA	SER
9	PHE	LYS	TRP	PHE	ASP	ASN	SER	ASN	MET	THR
10	PHE	ASP	LYS	TRP	THR	ASP	GLN	ASP	ASP	ASP
11	GLU	ASP	LEU	VAL	ASP	THR	CYS	ALA	PHE	LEU
12	HIS	ILE	LYS	THR	GLY	GLU	TRP	LYS	LYS	GLY
13	ASN	CYS	GLU	VAL	SER	SER	VAL	GLU	GLY	THR
14	LEU	CYS	LYS	THR	ALA	ILE	PRO	TYR	LYS	ARG