BMRB Entry 25810

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PDB ID: 2n7l
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR25810
MolProbity Validation Chart

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Title:
NMR structure of the N-domain of troponin C bound to the switch region of troponin I and the covalent levosimendan analog i9.
Deposition date:
2015-09-14
Original release date:
2016-07-20
Authors:
Pineda Sanabria, Sandra; Sykes, Brian; Robertson, Ian
Citation:

Citation: Pineda Sanabria, Sandra; Robertson, Ian; Sun, Yin-Biao; Irving, Malcolm; Sykes, Brian. "Troponin C with covalently bound levosimendan analog i9 enhances contraction in cardiac muscle fibers"  .

Assembly members:

Assembly members:
entity_1, polymer, 141 residues, 1702.936 Da.
entity_CA, non-polymer, 40.078 Da.

Natural source:

Natural source:   Common Name: not available   Taxonomy ID: not available   Superkingdom: not available   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET3

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: MHHHHHHGGLVPRGSMDDIY KAAVEQLTEEQKNEFKAAFD IFVLGAEDGSISTKELGKVM RMLGQNPTPEELQEMIDEVD EDGSGTVDFDEFLVMMVRXM KDDSENLYFQGRRVRISADA MMQALLGARAKESLDLRAHL K

Data sets:
Data typeCount
13C chemical shifts352
15N chemical shifts116
19F chemical shifts2
1H chemical shifts675

Additional metadata:

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Assembly:

Entity Assembly IDEntity NameEntity ID
1entity_11
2CALCIUM ION2

Entities:

Entity 1, entity_1 141 residues - 1702.936 Da.

MHHHHHHGGLVPRGSMDDIYKAAVEQLTEEQKNEFKAAFDIFVLGAEDGSISTKELGKVMRMLGQNPTPEELQEMIDEVD EDGSGTVDFDEFLVMMVR(4J4)MKDDSENLYFQGRRVRISADAMMQALLGARAKESLDLRAHLK Residues 1 to 7 are the His tag (Auth_seq_ID -15 to -9). Residues 8 to 15 are the thrombin cleavage site (Auth_seq_ID -8 to -1). Residues 16 to 104 are the N-domain of troponin C residues 1-89 (Auth_seq_ID 1 to 89). Residues 105 to 111 are the TEV cleavage site (Auth_seq_ID 90 to 96). Residues 112 to 141 are residues 144-173 of troponin I (Auth_seq_ID 97 to 126).

1   METHISHISHISHISHISHISGLYGLYLEU
2   VALPROARGGLYSERMETASPASPILETYR
3   LYSALAALAVALGLUGLNLEUTHRGLUGLU
4   GLNLYSASNGLUPHELYSALAALAPHEASP
5   ILEPHEVALLEUGLYALAGLUASPGLYSER
6   ILESERTHRLYSGLULEUGLYLYSVALMET
7   ARGMETLEUGLYGLNASNPROTHRPROGLU
8   GLULEUGLNGLUMETILEASPGLUVALASP
9   GLUASPGLYSERGLYTHRVALASPPHEASP
10   GLUPHELEUVALMETMETVALARG4J4MET
11   LYSASPASPSERGLUASNLEUTYRPHEGLN
12   GLYARGARGVALARGILESERALAASPALA
13   METMETGLNALALEULEUGLYALAARGALA
14   LYSGLUSERLEUASPLEUARGALAHISLEU
15   LYS

Entity 2, CALCIUM ION - Ca - 40.078 Da.

1   CA

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