BMRB Entry 25855

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PDB ID: 2n8m
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR25855
MolProbity Validation Chart

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Title:
Zipcode-binding-protein-1 KH3(DD)KH4 domains in complex with the RNA target UCGGACU
Deposition date:
2015-10-21
Original release date:
2017-01-19
Authors:
Nicastro, Giuseppe; Ramos, Andres; Candel, Adela; Hollingworth, David
Citation:

Citation: Hollingworth, David; Candel, Adela; Nicastro, Giuseppe; Martin, Stephen; Briata, Paola; Gherzi, Roberto; Ramos, Andres. "KH domains with impaired nucleic acid binding as a tool for functional analysis."  Nucleic Acids Res. 40, 6873-6886 (2012).
PubMed: 22547390

Assembly members:

Assembly members:
entity_1, polymer, 191 residues, 20613.682 Da.
entity_2, polymer, 8 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: E. coli   Taxonomy ID: 562   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: GAMGPSSVSGAAPFSSFMPP EQETVHVFIPAQAVGAIIGK KGQHIKQLSRFASASIKIAP PETPDSKVRMVVITGPPEAQ FKAQGRIYGKLKEENFFGPK EEVKLETHIRVPASAAGRVI GKGGKTVNELQNLTAAEVVV PRDQTPDENEQVIVKIIGHF YASQMAQRKIRDILAQVKQQ HQKGQSGQLQA
entity_2: UCGGACU

Data sets:
Data typeCount
13C chemical shifts403
1H chemical shifts941

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1entity_11
2entity_22

Entities:

Entity 1, entity_1 191 residues - 20613.682 Da.

1   GLYALAMETGLYPROSERSERVALSERGLY
2   ALAALAPROPHESERSERPHEMETPROPRO
3   GLUGLNGLUTHRVALHISVALPHEILEPRO
4   ALAGLNALAVALGLYALAILEILEGLYLYS
5   LYSGLYGLNHISILELYSGLNLEUSERARG
6   PHEALASERALASERILELYSILEALAPRO
7   PROGLUTHRPROASPSERLYSVALARGMET
8   VALVALILETHRGLYPROPROGLUALAGLN
9   PHELYSALAGLNGLYARGILETYRGLYLYS
10   LEULYSGLUGLUASNPHEPHEGLYPROLYS
11   GLUGLUVALLYSLEUGLUTHRHISILEARG
12   VALPROALASERALAALAGLYARGVALILE
13   GLYLYSGLYGLYLYSTHRVALASNGLULEU
14   GLNASNLEUTHRALAALAGLUVALVALVAL
15   PROARGASPGLNTHRPROASPGLUASNGLU
16   GLNVALILEVALLYSILEILEGLYHISPHE
17   TYRALASERGLNMETALAGLNARGLYSILE
18   ARGASPILELEUALAGLNVALLYSGLNGLN
19   HISGLNLYSGLYGLNSERGLYGLNLEUGLN
20   ALA

Entity 2, entity_2 8 residues - Formula weight is not available

1   UCGGACU