BMRB Entry 25924

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PDB ID: 2na2
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR25924
MolProbity Validation Chart

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Title:
Drosha Quad
Deposition date:
2015-12-17
Original release date:
2016-12-15
Authors:
Showalter, Scott; Sahu, Debashish; Kranick, Joshua
Citation:

Citation: Kranick, Joshua; Chadalavada, Durga; Sahu, Debashish; Showalter, Scott. "Engineering double-stranded RNA binding activity into the Drosha double-stranded RNA binding domain results in a loss of microRNA processing function"  PLoS ONE 12, e0182445-e0182445 (2017).
PubMed: 28792523

Assembly members:

Assembly members:
entity, polymer, 82 residues, 8973.405 Da.

Natural source:

Natural source:   Common Name: human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET49b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity: GPGNDPISQLQQCCLTLRTE GKEPDIPLYKTLQTVGPSHA RTYTVAVYFKGERIGCGKGP SKKQAKMGAAMDALEKYNFP QM

Data sets:
Data typeCount
13C chemical shifts298
15N chemical shifts67
1H chemical shifts356

Additional metadata:

  • Assembly
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Assembly:

Entity Assembly IDEntity NameEntity ID
1entity1

Entities:

Entity 1, entity 82 residues - 8973.405 Da.

1   GLYPROGLYASNASPPROILESERGLNLEU
2   GLNGLNCYSCYSLEUTHRLEUARGTHRGLU
3   GLYLYSGLUPROASPILEPROLEUTYRLYS
4   THRLEUGLNTHRVALGLYPROSERHISALA
5   ARGTHRTYRTHRVALALAVALTYRPHELYS
6   GLYGLUARGILEGLYCYSGLYLYSGLYPRO
7   SERLYSLYSGLNALALYSMETGLYALAALA
8   METASPALALEUGLULYSTYRASNPHEPRO
9   GLNMET

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