BMRB Entry 25958

Name: p63/p73 hetero-tetramerisation domain
Published: 2016-12-01

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PDB ID: 2nb1
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, SPARTA
BMRB Entry DOI: doi:10.13018/BMR25958
MolProbity Validation Chart

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

p63/p73 hetero-tetramerisation domain

Deposition date:

2016-01-19

Original release date:

2016-12-01

Authors:

Gebel, Jakob; Buchner, Lena; Loehr, Frank; Luh, Laura; Coutandin, Daniel; Guentert, Peter; Doetsch, Volker

Citation:

Citation: Gebel, Jakob; Luh, Laura; Coutandin, Daniel; Osterburg, Christian; Lohr, Frank; Schafer, Birgit; Frombach, Ann-Sophie; Sumyk, Manuela; Buchner, Lena; Krojer, Tobias; Salah, Eidarus; Mathea, Sebastian; Guntert, Peter; Knapp, Stefan; Dotsch, Volker. "Mechanism of TAp73 inhibition by Delta Np63 and structural basis of p63/p73 hetero-tetramerization" Cell Death Differ. 23, 1930-1940 (2016).
PubMed: 27716744

Assembly members:

Assembly members:
p63_tetramerization_domain, polymer, 60 residues, 7323.260 Da.
p73_tetramerization_domain, polymer, 50 residues, 6034.947 Da.

Natural source:

Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pBH4

Entity Sequences (FASTA):

Entity Sequences (FASTA):
p63_tetramerization_domain: SDDELLYLPVRGRETYEMLL EIKESLELMQYLPQHTIETY RQQQQQQHQHLLQKQTSIQS
p73_tetramerization_domain: GSDEDTYYLQVRGRKNFEIL MKLKESLELMELVPQPLVDS YRQQQQLLQR

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	950
15N chemical shifts	218
1H chemical shifts	1538

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	entity_1_1	1
2	entity_2_1	2
3	entity_1_2	1
4	entity_2_2	2

Entities:

Entity 1, entity_1_1 60 residues - 7323.260 Da.

Residue 1 represents a cloning artifact.

1	SER	ASP	ASP	GLU	LEU	LEU	TYR	LEU	PRO	VAL
2	ARG	GLY	ARG	GLU	THR	TYR	GLU	MET	LEU	LEU
3	GLU	ILE	LYS	GLU	SER	LEU	GLU	LEU	MET	GLN
4	TYR	LEU	PRO	GLN	HIS	THR	ILE	GLU	THR	TYR
5	ARG	GLN	GLN	GLN	GLN	GLN	GLN	HIS	GLN	HIS
6	LEU	LEU	GLN	LYS	GLN	THR	SER	ILE	GLN	SER

Entity 2, entity_2_1 50 residues - 6034.947 Da.

Residue 1-2 represent a cloning artifact.

1	GLY	SER	ASP	GLU	ASP	THR	TYR	TYR	LEU	GLN
2	VAL	ARG	GLY	ARG	LYS	ASN	PHE	GLU	ILE	LEU
3	MET	LYS	LEU	LYS	GLU	SER	LEU	GLU	LEU	MET
4	GLU	LEU	VAL	PRO	GLN	PRO	LEU	VAL	ASP	SER
5	TYR	ARG	GLN	GLN	GLN	GLN	LEU	LEU	GLN	ARG

Samples:

p63_13C15N_p73: p63 tetramerization domain, [U-100% 13C; U-100% 15N], 0.5 mM; p73 tetramerization domain 0.5 mM; HEPES 25 mM; sodium chloride 50 mM; H2O 95%; D2O 5%

p63_p73_13C15N: p63 tetramerization domain 0.5 mM; p73 tetramerization domain, [U-100% 13C; U-100% 15N], 0.5 mM; HEPES 25 mM; sodium chloride 50 mM; H2O 95%; D2O 5%

p63_13C_p63_15N_p73: p63 tetramerization domain, [U-100% 15N], 0.5 mM; p63 tetramerization domain, [U-100% 13C], 0.5 mM; p73 tetramerization domain 1 mM; HEPES 25 mM; sodium chloride 50 mM

p63_p73_13C_p73_15N: p63 tetramerization domain 1 mM; p73 tetramerization domain, [U-100% 15N], 0.5 mM; p73 tetramerization domain, [U-100% 13C], 0.5 mM; HEPES 25 mM; sodium chloride 50 mM

p63_15N_D2O: p63 tetramerization domain, [U-100% 15N], 0.5 mM; p73 tetramerization domain 0.5 mM; HEPES 25 mM; sodium chloride 50 mM

p73_15N_D2O: p63 tetramerization domain 0.5 mM; p73 tetramerization domain, [U-100% 15N], 0.5 mM; HEPES 25 mM; sodium chloride 50 mM

p63_13C_p63_15N_p73_13C_p73_15N: p63 tetramerization domain, [U-100% 15N], 0.5 mM; p63 tetramerization domain, [U-100% 13C], 0.5 mM; p73 tetramerization domain, [U-100% 15N], 0.5 mM; p73 tetramerization domain, [U-100% 13C], 0.5 mM; HEPES 25 mM; sodium chloride 50 mM

sample_conditions_1: ionic strength: 75 mM; pH: 7; pressure: 1 atm; temperature: 310 K

sample_conditions_2: ionic strength: 5 mM; pH: 6; pressure: 1 atm; temperature: 310 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D HNCACB	p63_13C15N_p73	isotropic	sample_conditions_1
3D HN(CA)CO	p63_13C15N_p73	isotropic	sample_conditions_1
3D 1H-15N NOESY	p63_13C15N_p73	isotropic	sample_conditions_1
3D HNCACB	p63_p73_13C15N	isotropic	sample_conditions_1
3D HN(CA)CO	p63_p73_13C15N	isotropic	sample_conditions_1
3D 1H-15N NOESY	p63_p73_13C15N	isotropic	sample_conditions_1
3D H(CCO)NH	p63_13C15N_p73	isotropic	sample_conditions_1
3D C(CO)NH	p63_13C15N_p73	isotropic	sample_conditions_1
3D H(CCO)NH	p63_p73_13C15N	isotropic	sample_conditions_1
3D C(CO)NH	p63_p73_13C15N	isotropic	sample_conditions_1
2D 1H-13C HSQC aromatic	p63_13C15N_p73	isotropic	sample_conditions_1
2D 1H-13C HSQC aromatic	p63_p73_13C15N	isotropic	sample_conditions_1
2D (H)CB(CG)CCH-TOCSY	p63_13C15N_p73	isotropic	sample_conditions_1
2D (H)CB(CG)CCH-TOCSY	p63_p73_13C15N	isotropic	sample_conditions_1
2D (HB)CB(CDCD)HD	p63_13C15N_p73	isotropic	sample_conditions_1
2D (HB)CB(CDCD)HD	p63_p73_13C15N	isotropic	sample_conditions_1
3D 1H-13C NOESY aromatic	p63_13C15N_p73	isotropic	sample_conditions_1
3D 1H-13C NOESY aromatic	p63_p73_13C15N	isotropic	sample_conditions_1
3D 13C/15N-filtered NOESY-[13C,1H]-HSQC	p63_13C15N_p73	isotropic	sample_conditions_1
3D 13C/15N-filtered NOESY-[13C,1H]-HSQC	p63_p73_13C15N	isotropic	sample_conditions_1
3D 13C/15N-filtered NOESY-[15N,1H]-TROSY	p63_13C15N_p73	isotropic	sample_conditions_1
3D 13C/15N-filtered NOESY-[15N,1H]-TROSY	p63_p73_13C15N	isotropic	sample_conditions_1
3D 1H-13C NOESY	p63_13C15N_p73	isotropic	sample_conditions_1
3D 1H-13C NOESY	p63_p73_13C15N	isotropic	sample_conditions_1
2D 1H-13C HSQC	p63_13C15N_p73	isotropic	sample_conditions_1
2D 1H-13C HSQC	p63_p73_13C15N	isotropic	sample_conditions_1
2D 1H-15N HSQC	p63_13C15N_p73	isotropic	sample_conditions_1
2D 1H-15N HSQC	p63_p73_13C15N	isotropic	sample_conditions_1
2D 1H-15N HSQC	p63_15N_D2O	isotropic	sample_conditions_1
2D 1H-15N HSQC	p73_15N_D2O	isotropic	sample_conditions_1
3D lr-HNCO	p63_13C_p63_15N_p73	isotropic	sample_conditions_1
3D lr-HNCO	p63_p73_13C_p73_15N	isotropic	sample_conditions_1
3D lr-HNCO	p63_13C_p63_15N_p73_13C_p73_15N	isotropic	sample_conditions_2

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SPARKY: Backbone or all simulated peaks