BMRB Entry 26016

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PDB ID: 2nce
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR26016
MolProbity Validation Chart

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Title:
Solution Structure of Ca2+-bound C2 domain from Protein Kinase C alpha in the form of complex with V5-pHM peptide
Deposition date:
2016-03-28
Original release date:
2017-03-24
Authors:
Yang, Yuan; Igumenova, Tatyana
Citation:

Citation: Yang, Yuan; Shu, Chang; Li, Pingwei; Igumenova, Tatyana. "Structural Basis of Protein Kinase Calpha Regulation by the C-Terminal Tail."  Biophys. J. 114, 1590-1603 (2018).
PubMed: 29642029

Assembly members:

Assembly members:
C2, polymer, 139 residues, 16240.596 Da.
CALCIUM ION, non-polymer, 40.078 Da.

Natural source:

Natural source:   Common Name: Rat   Taxonomy ID: 10116   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Rattus norvegicus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET-SUMO

Entity Sequences (FASTA):

Entity Sequences (FASTA):
C2: HTEKRGRIYLKAEVTDEKLH VTVRDAKNLIPMDPNGLSDP YVKLKLIPDPKNESKQKTKT IRSTLNPQWNESFTFKLKPS DKDRRLSVEIWDWDRTTRND FMGSLSFGVSELMKMPASGW YKLLNQEEGEYYNVPIPEG

Data sets:
Data typeCount
13C chemical shifts604
15N chemical shifts148
1H chemical shifts967

Additional metadata:

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Assembly:

Entity Assembly IDEntity NameEntity ID
1entity_11
2CALCIUM ION_12
3CALCIUM ION_22

Entities:

Entity 1, entity_1 139 residues - 16240.596 Da.

1   HISTHRGLULYSARGGLYARGILETYRLEU
2   LYSALAGLUVALTHRASPGLULYSLEUHIS
3   VALTHRVALARGASPALALYSASNLEUILE
4   PROMETASPPROASNGLYLEUSERASPPRO
5   TYRVALLYSLEULYSLEUILEPROASPPRO
6   LYSASNGLUSERLYSGLNLYSTHRLYSTHR
7   ILEARGSERTHRLEUASNPROGLNTRPASN
8   GLUSERPHETHRPHELYSLEULYSPROSER
9   ASPLYSASPARGARGLEUSERVALGLUILE
10   TRPASPTRPASPARGTHRTHRARGASNASP
11   PHEMETGLYSERLEUSERPHEGLYVALSER
12   GLULEUMETLYSMETPROALASERGLYTRP
13   TYRLYSLEULEUASNGLNGLUGLUGLYGLU
14   TYRTYRASNVALPROILEPROGLUGLY

Entity 2, CALCIUM ION_1 - Ca - 40.078 Da.

1   CA

Download HSQC peak lists in one of the following formats:
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SPARKY: Backbone or all simulated peaks