BMRB Entry 26052

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PDB ID: 2nd9
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR26052
MolProbity Validation Chart

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Title:
Solution structure of MapZ extracellular domain first subdomain
Deposition date:
2016-05-11
Original release date:
2016-06-27
Authors:
Jean, Nicolas; Manuse, Sylvie; Guinot, Megane; Bougault, Catherine; Grangeasse, Christophe; Simorre, Jean-Pierre
Citation:

Citation: Manuse, Sylvie; Jean, Nicolas; Guinot, Megane; Lavergne, Jean-Pierre; Laguri, Cedric; Bougault, Catherine; Van Nieuwenhze, Michael; Grangeasse, Christophe; Simorre, Jean-Pierre. "Structure-function analysis of the extracellular domain of the pneumococcal cell division site positioning protein MapZ"  Nat. commun. 7, 12071-12071 (2016).
PubMed: 27346279

Assembly members:

Assembly members:
MapZ_QG, polymer, 135 residues, 14847.4638 Da.

Natural source:

Natural source:   Common Name: firmicutes   Taxonomy ID: 1313   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Streptococcus pneumoniae

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pETPhos-MapZextra1

Entity Sequences (FASTA):

Entity Sequences (FASTA):
MapZ_QG: GHMQVARSTKEIETSQSTTA NQSDVDDFNTLYDAFYTNSN KTALKNSQFDKLSQLKTLLD KLEGSREHTLAKSKYDSLAT QIKAIQDVNAQFEKPAIVDG VLDTNAKAKSDAKFTDIKTG NTELDKVLDKAISLG

Data sets:
Data typeCount
13C chemical shifts557
15N chemical shifts137
1H chemical shifts841

Additional metadata:

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Assembly:

Entity Assembly IDEntity NameEntity ID
1MapZ QG1

Entities:

Entity 1, MapZ QG 135 residues - 14847.4638 Da.

Residues 1 to 3 correspond to the remnant of the Histidine-tag after cleavage.

1   GLYHISMETGLNVALALAARGSERTHRLYS
2   GLUILEGLUTHRSERGLNSERTHRTHRALA
3   ASNGLNSERASPVALASPASPPHEASNTHR
4   LEUTYRASPALAPHETYRTHRASNSERASN
5   LYSTHRALALEULYSASNSERGLNPHEASP
6   LYSLEUSERGLNLEULYSTHRLEULEUASP
7   LYSLEUGLUGLYSERARGGLUHISTHRLEU
8   ALALYSSERLYSTYRASPSERLEUALATHR
9   GLNILELYSALAILEGLNASPVALASNALA
10   GLNPHEGLULYSPROALAILEVALASPGLY
11   VALLEUASPTHRASNALALYSALALYSSER
12   ASPALALYSPHETHRASPILELYSTHRGLY
13   ASNTHRGLULEUASPLYSVALLEUASPLYS
14   ALAILESERLEUGLY

Related Database Links:

UNP Q8DR55
AlphaFold Q8DR55

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks