BMRB Entry 26317
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR26317
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Title: Backbone 1H, 13C, 15N, HA and Side Chain 13C Chemical Shift Assignments for RRM3 of PABPC1 PubMed: 35307347
Deposition date: 2021-09-07 Original release date: 2022-05-11
Authors: Sagae, Takeru; Yokogawa, Mariko; Osawa, Masanori
Citation: Sagae, Takeru; Yokogawa, Mariko; Sawazaki, Ryoichi; Ishii, Yuichiro; Hosoda, Nao; Hoshino, Shin-ichi; Imai, Sunsuke; Shimada, Ichio; Osawa, Masanori. "Paip2 competitively dissociates PABPC1 from poly(A) by initial access to RRM2 of the poly(A)-bound PABPC1" J. Biol. Chem. 298, 101844-101844 (2022).
Assembly members:
RRM3 of PABPC1, polymer, 104 residues, 11863 Da.
Natural source: Common Name: E. coli Taxonomy ID: 562 Superkingdom: Bacteria Kingdom: not available Genus/species: Escherichia coli
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pGEX-6p-1
Entity Sequences (FASTA):
RRM3 of PABPC1: GPLGSTNVYIKNFGEDMDDE
RLKDLFGKFGPALSVKVMTD
ESGKSKGFGFVSFERHEDAQ
KAVDEMNGKELNGKQIYVGR
AQKKVERQTELKRKFEQMKQ
DRIT
- assigned_chemical_shifts
Data type | Count |
13C chemical shifts | 268 |
15N chemical shifts | 97 |
1H chemical shifts | 188 |
Additional metadata:
Assembly:
Entity Assembly ID | Entity Name | Entity ID |
---|---|---|
1 | subunit 1 | 1 |
Entities:
Entity 1, subunit 1 104 residues - 11863 Da.
1 | GLY | PRO | LEU | GLY | SER | THR | ASN | VAL | TYR | ILE | ||||
2 | LYS | ASN | PHE | GLY | GLU | ASP | MET | ASP | ASP | GLU | ||||
3 | ARG | LEU | LYS | ASP | LEU | PHE | GLY | LYS | PHE | GLY | ||||
4 | PRO | ALA | LEU | SER | VAL | LYS | VAL | MET | THR | ASP | ||||
5 | GLU | SER | GLY | LYS | SER | LYS | GLY | PHE | GLY | PHE | ||||
6 | VAL | SER | PHE | GLU | ARG | HIS | GLU | ASP | ALA | GLN | ||||
7 | LYS | ALA | VAL | ASP | GLU | MET | ASN | GLY | LYS | GLU | ||||
8 | LEU | ASN | GLY | LYS | GLN | ILE | TYR | VAL | GLY | ARG | ||||
9 | ALA | GLN | LYS | LYS | VAL | GLU | ARG | GLN | THR | GLU | ||||
10 | LEU | LYS | ARG | LYS | PHE | GLU | GLN | MET | LYS | GLN | ||||
11 | ASP | ARG | ILE | THR |
Samples:
sample_1: RRM3 of PABPC1, [U-100% 13C; U-100% 15N], 431 uM; sodium phosphate 18 mM; sodium chloride 135 mM; H2O 50 M; D2O, [U-2H], 10%; DTT 0.9 mM
sample_conditions_1: pH: 6.5; pressure: 1 atm; temperature: 303 K
Experiments:
Name | Sample | Sample state | Sample conditions |
---|---|---|---|
2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
3D CC(CO)NH | sample_1 | isotropic | sample_conditions_1 |
3D 1H-15N NOESY | sample_1 | isotropic | sample_conditions_1 |
3D 1H-15N TOCSY | sample_1 | isotropic | sample_conditions_1 |
Software:
SPARKY v3.190 - chemical shift assignment
NMR spectrometers:
- Bruker Avance 800 MHz
- Bruker Avance 600 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts