BMRB Entry 26333

Name: Chemical shift assignments of the fusion protein consisting of the C-terminal deleted hepatitis B virus X protein BH3-like motif peptide and Bcl-xL
Published: 2023-07-19

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR26333

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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title: Chemical shift assignments of the fusion protein consisting of the C-terminal deleted hepatitis B virus X protein BH3-like motif peptide and Bcl-xL PubMed: 36044106

Deposition date: 2022-07-12 Original release date: 2023-07-19

Authors: Kusunoki, Hideki; Hamaguchi, Isao; Kobayashi, Naohiro; Nagata, Takashi

Citation: Kusunoki, Hideki; Hamaguchi, Isao; Kobayashi, Naohiro; Nagata, Takashi. "Chemical shift assignments of a fusion protein comprising the C-terminal-deleted hepatitis B virus X protein BH3-like motif peptide and Bcl-xL" Biomol. NMR Assign. 16, 357-361 (2022).

Assembly members:
HBx(101-120) and Bcl-xL fusion protein, polymer, 190 residues, Formula weight is not available

Natural source: Common Name: not available Taxonomy ID: 10407 Superkingdom: Viruses Kingdom: not available Genus/species: Orthohepadnavirus not available

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET15b

Entity Sequences (FASTA):
HBx(101-120) and Bcl-xL fusion protein: GSHMSAMSTTDLEAYFKDCV FKDWGSGSGSGSGSMSQSNR ELVVDFLSYKLSQKGYSWSQ FSDVEENRTEAPEGTESEAV KQALREAGDEFELRYRRAFS DLTSQLHITPGTAYQSFEQV VNELFRDGVNWGRIVAFFSF GGALCVESVDKEMQVLVSRI AAWMATYLNDHLEPWIQENG GWDTFVELYG

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	763
15N chemical shifts	184
1H chemical shifts	1175

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	HBx(101-120) and Bcl-xL fusion protein	1

Entities:

Entity 1, HBx(101-120) and Bcl-xL fusion protein 190 residues - Formula weight is not available

1	GLY	SER	HIS	MET	SER	ALA	MET	SER	THR	THR
2	ASP	LEU	GLU	ALA	TYR	PHE	LYS	ASP	CYS	VAL
3	PHE	LYS	ASP	TRP	GLY	SER	GLY	SER	GLY	SER
4	GLY	SER	GLY	SER	MET	SER	GLN	SER	ASN	ARG
5	GLU	LEU	VAL	VAL	ASP	PHE	LEU	SER	TYR	LYS
6	LEU	SER	GLN	LYS	GLY	TYR	SER	TRP	SER	GLN
7	PHE	SER	ASP	VAL	GLU	GLU	ASN	ARG	THR	GLU
8	ALA	PRO	GLU	GLY	THR	GLU	SER	GLU	ALA	VAL
9	LYS	GLN	ALA	LEU	ARG	GLU	ALA	GLY	ASP	GLU
10	PHE	GLU	LEU	ARG	TYR	ARG	ARG	ALA	PHE	SER
11	ASP	LEU	THR	SER	GLN	LEU	HIS	ILE	THR	PRO
12	GLY	THR	ALA	TYR	GLN	SER	PHE	GLU	GLN	VAL
13	VAL	ASN	GLU	LEU	PHE	ARG	ASP	GLY	VAL	ASN
14	TRP	GLY	ARG	ILE	VAL	ALA	PHE	PHE	SER	PHE
15	GLY	GLY	ALA	LEU	CYS	VAL	GLU	SER	VAL	ASP
16	LYS	GLU	MET	GLN	VAL	LEU	VAL	SER	ARG	ILE
17	ALA	ALA	TRP	MET	ALA	THR	TYR	LEU	ASN	ASP
18	HIS	LEU	GLU	PRO	TRP	ILE	GLN	GLU	ASN	GLY
19	GLY	TRP	ASP	THR	PHE	VAL	GLU	LEU	TYR	GLY

Samples:

sample_1: HBx(101-120)-linker-Bcl-xL, [U-99% 13C; U-99% 15N], 0.5 mM; potassium phosphate20 – 50 mM; sodium chloride 50 mM; H2O 95%; D2O, U-2H, 5%

sample_conditions_1: ionic strength: 0.05 M; pH: 6.8; pressure: 1 atm; temperature: 308 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
2D 1H-13C HSQC	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D HCCH-TOCSY	sample_1	isotropic	sample_conditions_1
3D C(CO)NH	sample_1	isotropic	sample_conditions_1

Software:

NMRDraw - chemical shift assignment

NMR spectrometers:

Bruker AVANCE III 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts

1	GLY	SER	HIS	MET	SER	ALA	MET	SER	THR	THR
2	ASP	LEU	GLU	ALA	TYR	PHE	LYS	ASP	CYS	VAL
3	PHE	LYS	ASP	TRP	GLY	SER	GLY	SER	GLY	SER
4	GLY	SER	GLY	SER	MET	SER	GLN	SER	ASN	ARG
5	GLU	LEU	VAL	VAL	ASP	PHE	LEU	SER	TYR	LYS
6	LEU	SER	GLN	LYS	GLY	TYR	SER	TRP	SER	GLN
7	PHE	SER	ASP	VAL	GLU	GLU	ASN	ARG	THR	GLU
8	ALA	PRO	GLU	GLY	THR	GLU	SER	GLU	ALA	VAL
9	LYS	GLN	ALA	LEU	ARG	GLU	ALA	GLY	ASP	GLU
10	PHE	GLU	LEU	ARG	TYR	ARG	ARG	ALA	PHE	SER
11	ASP	LEU	THR	SER	GLN	LEU	HIS	ILE	THR	PRO
12	GLY	THR	ALA	TYR	GLN	SER	PHE	GLU	GLN	VAL
13	VAL	ASN	GLU	LEU	PHE	ARG	ASP	GLY	VAL	ASN
14	TRP	GLY	ARG	ILE	VAL	ALA	PHE	PHE	SER	PHE
15	GLY	GLY	ALA	LEU	CYS	VAL	GLU	SER	VAL	ASP
16	LYS	GLU	MET	GLN	VAL	LEU	VAL	SER	ARG	ILE
17	ALA	ALA	TRP	MET	ALA	THR	TYR	LEU	ASN	ASP
18	HIS	LEU	GLU	PRO	TRP	ILE	GLN	GLU	ASN	GLY
19	GLY	TRP	ASP	THR	PHE	VAL	GLU	LEU	TYR	GLY

1	GLY	SER	HIS	MET	SER	ALA	MET	SER	THR	THR
2	ASP	LEU	GLU	ALA	TYR	PHE	LYS	ASP	CYS	VAL
3	PHE	LYS	ASP	TRP	GLY	SER	GLY	SER	GLY	SER
4	GLY	SER	GLY	SER	MET	SER	GLN	SER	ASN	ARG
5	GLU	LEU	VAL	VAL	ASP	PHE	LEU	SER	TYR	LYS
6	LEU	SER	GLN	LYS	GLY	TYR	SER	TRP	SER	GLN
7	PHE	SER	ASP	VAL	GLU	GLU	ASN	ARG	THR	GLU
8	ALA	PRO	GLU	GLY	THR	GLU	SER	GLU	ALA	VAL
9	LYS	GLN	ALA	LEU	ARG	GLU	ALA	GLY	ASP	GLU
10	PHE	GLU	LEU	ARG	TYR	ARG	ARG	ALA	PHE	SER
11	ASP	LEU	THR	SER	GLN	LEU	HIS	ILE	THR	PRO
12	GLY	THR	ALA	TYR	GLN	SER	PHE	GLU	GLN	VAL
13	VAL	ASN	GLU	LEU	PHE	ARG	ASP	GLY	VAL	ASN
14	TRP	GLY	ARG	ILE	VAL	ALA	PHE	PHE	SER	PHE
15	GLY	GLY	ALA	LEU	CYS	VAL	GLU	SER	VAL	ASP
16	LYS	GLU	MET	GLN	VAL	LEU	VAL	SER	ARG	ILE
17	ALA	ALA	TRP	MET	ALA	THR	TYR	LEU	ASN	ASP
18	HIS	LEU	GLU	PRO	TRP	ILE	GLN	GLU	ASN	GLY
19	GLY	TRP	ASP	THR	PHE	VAL	GLU	LEU	TYR	GLY

1	GLY	SER	HIS	MET	SER	ALA	MET	SER	THR	THR
2	ASP	LEU	GLU	ALA	TYR	PHE	LYS	ASP	CYS	VAL
3	PHE	LYS	ASP	TRP	GLY	SER	GLY	SER	GLY	SER
4	GLY	SER	GLY	SER	MET	SER	GLN	SER	ASN	ARG
5	GLU	LEU	VAL	VAL	ASP	PHE	LEU	SER	TYR	LYS
6	LEU	SER	GLN	LYS	GLY	TYR	SER	TRP	SER	GLN
7	PHE	SER	ASP	VAL	GLU	GLU	ASN	ARG	THR	GLU
8	ALA	PRO	GLU	GLY	THR	GLU	SER	GLU	ALA	VAL
9	LYS	GLN	ALA	LEU	ARG	GLU	ALA	GLY	ASP	GLU
10	PHE	GLU	LEU	ARG	TYR	ARG	ARG	ALA	PHE	SER
11	ASP	LEU	THR	SER	GLN	LEU	HIS	ILE	THR	PRO
12	GLY	THR	ALA	TYR	GLN	SER	PHE	GLU	GLN	VAL
13	VAL	ASN	GLU	LEU	PHE	ARG	ASP	GLY	VAL	ASN
14	TRP	GLY	ARG	ILE	VAL	ALA	PHE	PHE	SER	PHE
15	GLY	GLY	ALA	LEU	CYS	VAL	GLU	SER	VAL	ASP
16	LYS	GLU	MET	GLN	VAL	LEU	VAL	SER	ARG	ILE
17	ALA	ALA	TRP	MET	ALA	THR	TYR	LEU	ASN	ASP
18	HIS	LEU	GLU	PRO	TRP	ILE	GLN	GLU	ASN	GLY
19	GLY	TRP	ASP	THR	PHE	VAL	GLU	LEU	TYR	GLY