BMRB Entry 26518
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR26518
MolProbity Validation Chart
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
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Title: NMR solution structure of the putative transfer protein TraH from Gram-positive conjugative plasmid pIP501 PubMed: 27103580
Deposition date: 2015-02-23 Original release date: 2016-07-13
Authors: Meyer, Niels Helge; Fercher, C.; Zangger, K.; Keller, W.
Citation: Fercher, Christian; Probst, Ines; Kohler, Verena; Goessweiner-Mohr, Nikolaus; Arends, Karsten; Grohmann, Elisabeth; Zangger, Klaus; Meyer, N. Helge; Keller, Walter. "VirB8-like protein TraH is crucial for DNA transfer in Enterococcus faecalis" Sci. Rep. 6, 24643-24643 (2016).
Assembly members:
TRAH, polymer, 128 residues, 14896.3656 Da.
Natural source: Common Name: Enterococcus faecalis Taxonomy ID: 1351 Superkingdom: Bacteria Kingdom: not available Genus/species: Enterococcus faecalis
Experimental source: Production method: recombinant technology Host organism: ESCHERICHIA COLI BL21 Vector: na
Entity Sequences (FASTA):
TRAH: SNTNQSESEKIIKEFYKTVY
NYEKSQKEISMTTVKELATD
NVYQELQNEINVNNSYSPQQ
NTIQKSSVNENEIKILAYES
KDNSQQYLVTAPIHQVFNGT
KNDFEINQLIQIKNQKITQR
TTIQLGEE
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 563 |
| 15N chemical shifts | 147 |
| 1H chemical shifts | 925 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | TRAH | 1 |
Entities:
Entity 1, TRAH 128 residues - 14896.3656 Da.
| 1 | SER | ASN | THR | ASN | GLN | SER | GLU | SER | GLU | LYS | ||||
| 2 | ILE | ILE | LYS | GLU | PHE | TYR | LYS | THR | VAL | TYR | ||||
| 3 | ASN | TYR | GLU | LYS | SER | GLN | LYS | GLU | ILE | SER | ||||
| 4 | MET | THR | THR | VAL | LYS | GLU | LEU | ALA | THR | ASP | ||||
| 5 | ASN | VAL | TYR | GLN | GLU | LEU | GLN | ASN | GLU | ILE | ||||
| 6 | ASN | VAL | ASN | ASN | SER | TYR | SER | PRO | GLN | GLN | ||||
| 7 | ASN | THR | ILE | GLN | LYS | SER | SER | VAL | ASN | GLU | ||||
| 8 | ASN | GLU | ILE | LYS | ILE | LEU | ALA | TYR | GLU | SER | ||||
| 9 | LYS | ASP | ASN | SER | GLN | GLN | TYR | LEU | VAL | THR | ||||
| 10 | ALA | PRO | ILE | HIS | GLN | VAL | PHE | ASN | GLY | THR | ||||
| 11 | LYS | ASN | ASP | PHE | GLU | ILE | ASN | GLN | LEU | ILE | ||||
| 12 | GLN | ILE | LYS | ASN | GLN | LYS | ILE | THR | GLN | ARG | ||||
| 13 | THR | THR | ILE | GLN | LEU | GLY | GLU | GLU |
Samples:
sample_1: TRAH, [U-13C; U-15N], 0.5 mM; PO4 50 mM; NaCl 100 mM
sample_conditions_1: ionic strength: 200.000 mM; pH: 6.500; pressure: 1.000 atm; temperature: 298.000 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| NOESY | sample_1 | solution | sample_conditions_1 |
Software:
AutoDep v4.3, PDBe - chemical shift assignment
CNS vany, Brunger, Adams, Clore, Gros, Nilges and Read - chemical shift assignment
CYANA vany, Guntert, Mumenthaler and Wuthrich - chemical shift assignment
RECOORD_CNS vany, A.J. NEDERVEEN, J.F. DORELEIJERS, W.F. - chemical shift assignment
NMR spectrometers:
- Bruker Avance 700 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts