BMRB Entry 26548

Name: Solid-state NMR sequential assignments of the N-terminal domain of HpDnaB helicase
Published: 2015-08-24

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR26548

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Solid-state NMR sequential assignments of the N-terminal domain of HpDnaB helicase

Deposition date:

2015-03-24

Original release date:

2015-08-24

Authors:

Wiegand, Thomas; Gardiennet, Carole; Meier, Beat; Bockmann, Anja; Cadalbert, Riccardo; Lacabanne, Denis; Kunert, Britta; Guentert, Peter; Terradot, Laurent; Ravotti, Francesco

Citation:

Citation: Wiegand, Thomas; Gardiennet, Carole; Ravotti, Francesco; Cadalbert, Riccardo; Lacabanne, Denis; Kunert, Britta; Guentert, Peter; Terradot, Laurent; Bockmann, Anja; Meier, Beat. "Solid-state NMR sequential assignments of the N-terminal domain of HpDnaB helicase" Biomol. NMR Assign. 10, 13-23 (2016).
PubMed: 26280528

Assembly members:

Assembly members:
HpDnaB_Nter, polymer, 159 residues, Formula weight is not available

Natural source:

Natural source: Common Name: H. pylori Taxonomy ID: 210 Superkingdom: Bacteria Kingdom: not available Genus/species: Helicobacter pylori

Experimental source:

Experimental source: Production method: purified from the natural source Host organism: Helicobacter pylori Vector: pET151

Entity Sequences (FASTA):

Entity Sequences (FASTA):
HpDnaB_Nter: GIDPFTMDHLKHLQQLQNIE RIVLSGIVLANHKIEEVHSV LEPSDFYYPPNGLFFEIALK LHEEDCPIDENFIRQKMPKD KQIKEEDLVAIFAASPIDNI EAYVEEIKNASIKRKLFGLA NTIREQALESAQKSSDILGA VEREVYALLNGSTIEGFRN

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1
- assigned_chem_shift_list_2

Data type	Count
13C chemical shifts	499
15N chemical shifts	112

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
Hide all

Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	HpDnaB_Nter_chainA	1
2	HpDnaB_Nter_chainB	1

Entities:

Entity 1, HpDnaB_Nter_chainA 159 residues - Formula weight is not available

1

GLY

ILE

ASP

PRO

PHE

THR

MET

ASP

HIS

LEU

2

LYS

HIS

LEU

GLN

LEU

GLN

ASN

ILE

GLU

3

ARG

ILE

VAL

LEU

SER

GLY

ILE

VAL

LEU

ALA

4

ASN

HIS

LYS

ILE

GLU

VAL

HIS

SER

VAL

5

LEU

GLU

PRO

SER

ASP

PHE

TYR

PRO

6

ASN

GLY

LEU

PHE

GLU

ILE

ALA

LEU

LYS

7

LEU

HIS

GLU

ASP

CYS

PRO

ILE

ASP

GLU

8

ASN

PHE

ILE

ARG

GLN

LYS

MET

PRO

LYS

ASP

9

LYS

GLN

ILE

LYS

GLU

ASP

LEU

VAL

ALA

10

ILE

PHE

ALA

SER

PRO

ILE

ASP

ASN

ILE

11

GLU

ALA

TYR

VAL

GLU

ILE

LYS

ASN

ALA

12

SER

ILE

LYS

ARG

LYS

LEU

PHE

GLY

LEU

ALA

13

ASN

THR

ILE

ARG

GLU

GLN

ALA

LEU

GLU

SER

14

ALA

GLN

LYS

SER

ASP

ILE

LEU

GLY

ALA

15

VAL

GLU

ARG

GLU

VAL

TYR

ALA

LEU

ASN

16

GLY

SER

THR

ILE

GLU

GLY

PHE

ARG

ASN

Samples:

sample_1: HpDnaB_Nter, [U-100% 13C; U-100% 15N], 23.5 mg/mL; TRIS-HCl 50 mM; NaCl 100 mM; HEPES 100 mM; sodium azide 0.1%; glycerol 10%

sample_conditions_1: pH: 6.5; pressure: 1 atm; temperature: 293 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 13C-13C DARR 10ms	sample_1	isotropic	sample_conditions_1
2D 13C-13C DARR 20ms	sample_1	isotropic	sample_conditions_1
2D 13C-13C DARR 50ms	sample_1	isotropic	sample_conditions_1
2D 13C-13C DARR 200ms	sample_1	isotropic	sample_conditions_1
2D 15N-13C NCA	sample_1	isotropic	sample_conditions_1
3D 15N-13C-13C NCACB	sample_1	isotropic	sample_conditions_1
3D 15N-13C-13C NCACX	sample_1	isotropic	sample_conditions_1
3D 15N-13C-13C NCAOCX	sample_1	isotropic	sample_conditions_1
3D 13C-15N-13C CANCO	sample_1	isotropic	sample_conditions_1
3D 15N-13C-13C NcoCACB	sample_1	isotropic	sample_conditions_1
3D 15N-13C-13C CANcoCA	sample_1	isotropic	sample_conditions_1
3D 15N-13C-13C NCaCBCX	sample_1	isotropic	sample_conditions_1
3D 13C-13C-13C CCC	sample_1	isotropic	sample_conditions_1

Software:

TOPSPIN_v._3.2, Bruker Biospin - data analysis, processing

CCPN_v._2.4.1, Fogh et al. - chemical shift assignment, data analysis, peak picking

NMR spectrometers:

Bruker Avance 800 MHz
Bruker Avance 850 MHz