BMRB Entry 26706
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR26706
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Title: Backbone 13C, and 15 N Chemical Shift Assignments for fibrillar Islet Amyloid Polypeptide (IAPP) PubMed: 27607147
Deposition date: 2015-11-12 Original release date: 2016-08-08
Authors: Weirich, Franziska; Heise, Henrike; Mirecka, Ewa; Gremer, Lothar; Hoyer, Wolfgang; Schiefer, Stephanie
Citation: Weirich, Franziska; Mirecka, Ewa; Schiefer, Stephanie; Gremer, Lothar; Hoyer, Wolfgang; Heise, Henrike. "Structural Characterization of Fibrils from Recombinant Human Islet Amyloid Polypeptide by Solid-State NMR: The Central FGAILS Segment Is Part of the beta-Sheet Core" PLOS One 11, e0161243-e0161243 (2016).
Assembly members:
IAPP, polymer, 37 residues, 3906.3 Da.
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET302/NT-His
Entity Sequences (FASTA):
IAPP: KCNTATCATQRLANFLVHSS
NNFGAILSSTNVGSNTY
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 109 |
| 15N chemical shifts | 37 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | recombinant fibrillar hIAPP | 1 |
Entities:
Entity 1, recombinant fibrillar hIAPP 37 residues - 3906.3 Da.
Full length human IAPP.
| 1 | LYS | CYS | ASN | THR | ALA | THR | CYS | ALA | THR | GLN | ||||
| 2 | ARG | LEU | ALA | ASN | PHE | LEU | VAL | HIS | SER | SER | ||||
| 3 | ASN | ASN | PHE | GLY | ALA | ILE | LEU | SER | SER | THR | ||||
| 4 | ASN | VAL | GLY | SER | ASN | THR | TYR |
Samples:
sample_1: IAPP, [U-100% 13C; U-100% 15N], 110 mM
sample_conditions_1: pH: 7.4; pressure: 1 atm; temperature: 273 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 13C-13C PDSD | sample_1 | isotropic | sample_conditions_1 |
| 3D NCOCX | sample_1 | isotropic | sample_conditions_1 |
| 3D NCaCB | sample_1 | isotropic | sample_conditions_1 |
| 3D NCaCX | sample_1 | isotropic | sample_conditions_1 |
| 1D INEPT | sample_1 | isotropic | sample_conditions_1 |
| 2D DQSQ SPC5_3 | sample_1 | isotropic | sample_conditions_1 |
Software:
CcpNMR v2.4.2, CCPN - chemical shift assignment
VNMRJ, Varian - collection
NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing
NMR spectrometers:
- Oxford AS600 600 MHz
- Varian 600/54 Premium Shielded 600 MHz
- Oxford 2.2K 800/63 800 MHz