BMRB Entry 26767
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR26767
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Title: Backbone 1H, 15N and 13C assignments of the catalytic domain of human NSD1 PubMed: 27356987
Deposition date: 2016-03-23 Original release date: 2016-04-22
Authors: Amin, Nader; Nietlispach, Daniel; Coyle, Joe; Williams, Glyn; Chiarparin, Elisabetta
Citation: Amin, Nader; Nietlispach, Daniel; Qamar, Seema; Coyle, Joe; Chiarparin, Elisabetta; Williams, Glyn. "NMR backbone resonance assignment and solution secondary structure determination of human NSD1 and NSD2" Biomol. NMR Assign. 10, 315-320 (2016).
Assembly members:
NSD1, polymer, 232 residues, Formula weight is not available
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET23b
Entity Sequences (FASTA):
NSD1: MKELRQLQEDRKNDKKPPPY
KHIKVNRPIGRVQIFTADLS
EIPRCNCKATDENPCGIDSE
CINRMLLYECHPTVCPAGGR
CQNQCFSKRQYPEVEIFRTL
QRGWGLRTKTDIKKGEFVNE
YVGELIDEEECRARIRYAQE
HDITNFYMLTLDKDRIIDAG
PKGNYARFMNHCCQPNCETQ
KWSVNGDTRVGLFALSDIKA
GTELTFNYNLECLGNGKTVC
KCGAPNCSGFLG
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 612 |
| 15N chemical shifts | 212 |
| 1H chemical shifts | 212 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | NSD1 | 1 |
Entities:
Entity 1, NSD1 232 residues - Formula weight is not available
| 1 | MET | LYS | GLU | LEU | ARG | GLN | LEU | GLN | GLU | ASP | ||||
| 2 | ARG | LYS | ASN | ASP | LYS | LYS | PRO | PRO | PRO | TYR | ||||
| 3 | LYS | HIS | ILE | LYS | VAL | ASN | ARG | PRO | ILE | GLY | ||||
| 4 | ARG | VAL | GLN | ILE | PHE | THR | ALA | ASP | LEU | SER | ||||
| 5 | GLU | ILE | PRO | ARG | CYS | ASN | CYS | LYS | ALA | THR | ||||
| 6 | ASP | GLU | ASN | PRO | CYS | GLY | ILE | ASP | SER | GLU | ||||
| 7 | CYS | ILE | ASN | ARG | MET | LEU | LEU | TYR | GLU | CYS | ||||
| 8 | HIS | PRO | THR | VAL | CYS | PRO | ALA | GLY | GLY | ARG | ||||
| 9 | CYS | GLN | ASN | GLN | CYS | PHE | SER | LYS | ARG | GLN | ||||
| 10 | TYR | PRO | GLU | VAL | GLU | ILE | PHE | ARG | THR | LEU | ||||
| 11 | GLN | ARG | GLY | TRP | GLY | LEU | ARG | THR | LYS | THR | ||||
| 12 | ASP | ILE | LYS | LYS | GLY | GLU | PHE | VAL | ASN | GLU | ||||
| 13 | TYR | VAL | GLY | GLU | LEU | ILE | ASP | GLU | GLU | GLU | ||||
| 14 | CYS | ARG | ALA | ARG | ILE | ARG | TYR | ALA | GLN | GLU | ||||
| 15 | HIS | ASP | ILE | THR | ASN | PHE | TYR | MET | LEU | THR | ||||
| 16 | LEU | ASP | LYS | ASP | ARG | ILE | ILE | ASP | ALA | GLY | ||||
| 17 | PRO | LYS | GLY | ASN | TYR | ALA | ARG | PHE | MET | ASN | ||||
| 18 | HIS | CYS | CYS | GLN | PRO | ASN | CYS | GLU | THR | GLN | ||||
| 19 | LYS | TRP | SER | VAL | ASN | GLY | ASP | THR | ARG | VAL | ||||
| 20 | GLY | LEU | PHE | ALA | LEU | SER | ASP | ILE | LYS | ALA | ||||
| 21 | GLY | THR | GLU | LEU | THR | PHE | ASN | TYR | ASN | LEU | ||||
| 22 | GLU | CYS | LEU | GLY | ASN | GLY | LYS | THR | VAL | CYS | ||||
| 23 | LYS | CYS | GLY | ALA | PRO | ASN | CYS | SER | GLY | PHE | ||||
| 24 | LEU | GLY |
Samples:
sample_1: NSD1, [U-98% 13C; U-98% 15N], 0.2 mM; MES 50 mM; sodium chloride 450 mM; TCEP 2 mM; S-adenosyl methionine 2 mM; sodium azide 0.01 % w/v; D2O 10 % v/v
sample_conditions_1: ionic strength: 450 mM; pH: 6.5; pressure: 1 atm; temperature: 310 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCA | sample_1 | isotropic | sample_conditions_1 |
| 3D HN(CO)CA | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
| 3D HN(COCA)CB | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCO | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-15N NOESY | sample_1 | isotropic | sample_conditions_1 |
Software:
CCPN_Analysis, CCPN - chemical shift assignment
NMR spectrometers:
- Bruker Avance 600 MHz
- Bruker Avance 500 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts