BMRB Entry 26879

Name: Solid-state NMR 13C and 15N chemical shift assignments for AL-09 VL immunoglobulin light chain fibrils
Published: 2017-02-16

Chem Shift validation: AVS_anomalous, AVS_full
BMRB Entry DOI: doi:10.13018/BMR26879

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Solid-state NMR 13C and 15N chemical shift assignments for AL-09 VL immunoglobulin light chain fibrils

Deposition date:

2016-08-16

Original release date:

2017-02-16

Authors:

Piehl, Dennis; Blancas-Mejia, Luis; Ramirez-Alvarado, Marina; Rienstra, Chad

Citation:

Citation: Piehl, Dennis; Blancas-Mejia, Luis; Ramirez-Alvarado, Marina; Rienstra, Chad. "Solid-state NMR chemical shift assignments for AL-09 VL immunoglobulin light chain fibrils" Biomol. NMR Assignments 11, 45-50 (2017).
PubMed: 27771830

Assembly members:

Assembly members:
AL-09_VL_fibrils, polymer, 110 residues, 12121.3024 Da.

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET12a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
AL-09_VL_fibrils: STDIQMTQSPSSLSASVGDR VTITCQASQDINNYLIWYQQ KPGQAPKLLIYDASTLETGV PSRFSGSGSGTEFTFTISSL QPEDLATYHCQQYDNLPYTF GQGTKLEIKR

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	188
15N chemical shifts	54

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	AL-09 VL light chain protein	1

Entities:

Entity 1, AL-09 VL light chain protein 110 residues - 12121.3024 Da.

Variable domain sequence of AL-09 light chain protein. Contains two N-terminal residue purification artifacts, S-1 and T0.

1	SER	THR	ASP	ILE	GLN	MET	THR	GLN	SER	PRO
2	SER	SER	LEU	SER	ALA	SER	VAL	GLY	ASP	ARG
3	VAL	THR	ILE	THR	CYS	GLN	ALA	SER	GLN	ASP
4	ILE	ASN	ASN	TYR	LEU	ILE	TRP	TYR	GLN	GLN
5	LYS	PRO	GLY	GLN	ALA	PRO	LYS	LEU	LEU	ILE
6	TYR	ASP	ALA	SER	THR	LEU	GLU	THR	GLY	VAL
7	PRO	SER	ARG	PHE	SER	GLY	SER	GLY	SER	GLY
8	THR	GLU	PHE	THR	PHE	THR	ILE	SER	SER	LEU
9	GLN	PRO	GLU	ASP	LEU	ALA	THR	TYR	HIS	CYS
10	GLN	GLN	TYR	ASP	ASN	LEU	PRO	TYR	THR	PHE
11	GLY	GLN	GLY	THR	LYS	LEU	GLU	ILE	LYS	ARG

Name	Sample	Sample state	Sample conditions
NCACX 25 ms DARR	sample_1	isotropic	sample_conditions_1
NCOCX 25 ms DARR	sample_1	isotropic	sample_conditions_1
CANcoCX 25 ms DARR	sample_1	isotropic	sample_conditions_1
CANCO	sample_1	isotropic	sample_conditions_1
NCA(CB)CO SPC7	sample_1	isotropic	sample_conditions_1
NCOCA SPC7	sample_1	isotropic	sample_conditions_1
NCACX 90 ms DARR	sample_1	isotropic	sample_conditions_1
NCOCX 90 ms DARR	sample_1	isotropic	sample_conditions_1
CC 40 ms DARR	sample_1	isotropic	sample_conditions_1
CCC SPC7 + 50 ms DARR	sample_1	isotropic	sample_conditions_1

UNP	A2NI60 P01594
PDB	3CDY
AlphaFold	A2NI60 P01603

BMRB Entry 26879

Additional metadata:

Assembly:

Entities:

Samples:

Experiments:

Software:

NMR spectrometers:

Related Database Links:

1	SER	THR	ASP	ILE	GLN	MET	THR	GLN	SER	PRO
2	SER	SER	LEU	SER	ALA	SER	VAL	GLY	ASP	ARG
3	VAL	THR	ILE	THR	CYS	GLN	ALA	SER	GLN	ASP
4	ILE	ASN	ASN	TYR	LEU	ILE	TRP	TYR	GLN	GLN
5	LYS	PRO	GLY	GLN	ALA	PRO	LYS	LEU	LEU	ILE
6	TYR	ASP	ALA	SER	THR	LEU	GLU	THR	GLY	VAL
7	PRO	SER	ARG	PHE	SER	GLY	SER	GLY	SER	GLY
8	THR	GLU	PHE	THR	PHE	THR	ILE	SER	SER	LEU
9	GLN	PRO	GLU	ASP	LEU	ALA	THR	TYR	HIS	CYS
10	GLN	GLN	TYR	ASP	ASN	LEU	PRO	TYR	THR	PHE
11	GLY	GLN	GLY	THR	LYS	LEU	GLU	ILE	LYS	ARG

1	SER	THR	ASP	ILE	GLN	MET	THR	GLN	SER	PRO
2	SER	SER	LEU	SER	ALA	SER	VAL	GLY	ASP	ARG
3	VAL	THR	ILE	THR	CYS	GLN	ALA	SER	GLN	ASP
4	ILE	ASN	ASN	TYR	LEU	ILE	TRP	TYR	GLN	GLN
5	LYS	PRO	GLY	GLN	ALA	PRO	LYS	LEU	LEU	ILE
6	TYR	ASP	ALA	SER	THR	LEU	GLU	THR	GLY	VAL
7	PRO	SER	ARG	PHE	SER	GLY	SER	GLY	SER	GLY
8	THR	GLU	PHE	THR	PHE	THR	ILE	SER	SER	LEU
9	GLN	PRO	GLU	ASP	LEU	ALA	THR	TYR	HIS	CYS
10	GLN	GLN	TYR	ASP	ASN	LEU	PRO	TYR	THR	PHE
11	GLY	GLN	GLY	THR	LYS	LEU	GLU	ILE	LYS	ARG

1	SER	THR	ASP	ILE	GLN	MET	THR	GLN	SER	PRO
2	SER	SER	LEU	SER	ALA	SER	VAL	GLY	ASP	ARG
3	VAL	THR	ILE	THR	CYS	GLN	ALA	SER	GLN	ASP
4	ILE	ASN	ASN	TYR	LEU	ILE	TRP	TYR	GLN	GLN
5	LYS	PRO	GLY	GLN	ALA	PRO	LYS	LEU	LEU	ILE
6	TYR	ASP	ALA	SER	THR	LEU	GLU	THR	GLY	VAL
7	PRO	SER	ARG	PHE	SER	GLY	SER	GLY	SER	GLY
8	THR	GLU	PHE	THR	PHE	THR	ILE	SER	SER	LEU
9	GLN	PRO	GLU	ASP	LEU	ALA	THR	TYR	HIS	CYS
10	GLN	GLN	TYR	ASP	ASN	LEU	PRO	TYR	THR	PHE
11	GLY	GLN	GLY	THR	LYS	LEU	GLU	ILE	LYS	ARG