Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR26909
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Citation: Alyami, Esmael; Rizzo, Alessandro; Beuning, Penny; Korzhnev, Dmitry. "NMR resonance assignments for the N-terminal domain of the delta subunit of the E. coli gamma clamp loader complex" Biomol. NMR Assignments 11, 169-173 (2017).
PubMed: 28265855
Assembly members:
E_coli_DNA_polymerase_III_delta_subunit_(residues_1-140), polymer, 143 residues, Formula weight is not available
Natural source: Common Name: enterobacteria Taxonomy ID: 562 Superkingdom: Bacteria Kingdom: not available Genus/species: Escherichia coli
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET28b+
Entity Sequences (FASTA):
E_coli_DNA_polymerase_III_delta_subunit_(residues_1-140): GSHMIRLYPEQLRAQLNEGL
RAAYLLLGNDPLLLQESQDA
VRQVAAAQGFEEHHTFSIDP
NTDWNAIFSLCQAMSLFASR
QTLLLLLPENGPNAAINEQL
LTLTGLLHDDLLLIVRGNKL
SKAQENAAWFTALANRSVQV
TCQ
Data type | Count |
13C chemical shifts | 587 |
15N chemical shifts | 148 |
1H chemical shifts | 953 |
Entity Assembly ID | Entity Name | Entity ID |
---|---|---|
1 | E coli DNA polymerase III delta subunit (residues 1-140) | 1 |
Entity 1, E coli DNA polymerase III delta subunit (residues 1-140) 143 residues - Formula weight is not available
GSH are cloning artifacts. The native residues are M1-Q140
1 | GLY | SER | HIS | MET | ILE | ARG | LEU | TYR | PRO | GLU | ||||
2 | GLN | LEU | ARG | ALA | GLN | LEU | ASN | GLU | GLY | LEU | ||||
3 | ARG | ALA | ALA | TYR | LEU | LEU | LEU | GLY | ASN | ASP | ||||
4 | PRO | LEU | LEU | LEU | GLN | GLU | SER | GLN | ASP | ALA | ||||
5 | VAL | ARG | GLN | VAL | ALA | ALA | ALA | GLN | GLY | PHE | ||||
6 | GLU | GLU | HIS | HIS | THR | PHE | SER | ILE | ASP | PRO | ||||
7 | ASN | THR | ASP | TRP | ASN | ALA | ILE | PHE | SER | LEU | ||||
8 | CYS | GLN | ALA | MET | SER | LEU | PHE | ALA | SER | ARG | ||||
9 | GLN | THR | LEU | LEU | LEU | LEU | LEU | PRO | GLU | ASN | ||||
10 | GLY | PRO | ASN | ALA | ALA | ILE | ASN | GLU | GLN | LEU | ||||
11 | LEU | THR | LEU | THR | GLY | LEU | LEU | HIS | ASP | ASP | ||||
12 | LEU | LEU | LEU | ILE | VAL | ARG | GLY | ASN | LYS | LEU | ||||
13 | SER | LYS | ALA | GLN | GLU | ASN | ALA | ALA | TRP | PHE | ||||
14 | THR | ALA | LEU | ALA | ASN | ARG | SER | VAL | GLN | VAL | ||||
15 | THR | CYS | GLN |
sample_1: E coli DNA polymerase III delta subunit (residues 1-140), [U-13C; U-15N], 1.5 mM; D2O 7.5%; sodium phosphate 20 mM; NaCl 100 mM; DTT 10 mM; EDTA 1 mM; sodium azide 0.05 % v/v; Halt protease inhibitor cocktail 0.33 % v/v
sample_conditions_1: ionic strength: 100 mM; pH: 6.8; pressure: 1 atm; temperature: 273 K
Name | Sample | Sample state | Sample conditions |
---|---|---|---|
2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
2D 1H-13C HSQC | sample_1 | isotropic | sample_conditions_1 |
3D HNCO | sample_1 | isotropic | sample_conditions_1 |
3D HNCA | sample_1 | isotropic | sample_conditions_1 |
3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
3D HN(Ca)CO | sample_1 | isotropic | sample_conditions_1 |
3D HBHA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
3D HCCH-TOCSY | sample_1 | isotropic | sample_conditions_1 |
3D CCH-TOCSY | sample_1 | isotropic | sample_conditions_1 |
Analysis, CCPN - chemical shift assignment
NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing
VNMRJ, Varian - collection
TALOS, Cornilescu, Delaglio and Bax - data analysis
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated peaks
SPARKY: Backbone
or all simulated peaks