BMRB Entry 26910

Name: 13C and 15N chemical shift assignment of the Y21M mutation of fd filamentous phage
Published: 2017-07-19

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR26910

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

13C and 15N chemical shift assignment of the Y21M mutation of fd filamentous phage

Deposition date:

2016-09-28

Original release date:

2017-07-19

Authors:

Goldbourt, Amir; Abramov, Gili

Citation:

Citation: Abramov, Gili; Shaharabani, Rona; Morag, Omry; Avinery, Ram; Haimovich, Anat; Oz, Inbal; Beck, Roy; Goldbourt, Amir. "Structural Effects of Single Mutations in a Filamentous Viral Capsid Across Multiple Length Scales" Biomacromolecules 18, 2258-2266 (2017).
PubMed: 28657731

Assembly members:

Assembly members:
fd-y21m_phage, polymer, 50 residues, Formula weight is not available

Natural source:

Natural source: Common Name: E. coli Taxonomy ID: 562 Superkingdom: Bacteria Kingdom: not available Genus/species: Escherichia coli

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli

Entity Sequences (FASTA):

Entity Sequences (FASTA):
fd-y21m_phage: AEGDDPAKAAFDSLQASATE MIGYAWAMVVVIVGATIGIK LFKKFTSKAS

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	221
15N chemical shifts	52

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	y21m protein coat monomer	1

Entities:

Entity 1, y21m protein coat monomer 50 residues - Formula weight is not available

1	ALA	GLU	GLY	ASP	ASP	PRO	ALA	LYS	ALA	ALA
2	PHE	ASP	SER	LEU	GLN	ALA	SER	ALA	THR	GLU
3	MET	ILE	GLY	TYR	ALA	TRP	ALA	MET	VAL	VAL
4	VAL	ILE	VAL	GLY	ALA	THR	ILE	GLY	ILE	LYS
5	LEU	PHE	LYS	LYS	PHE	THR	SER	LYS	ALA	SER

Samples:

sample_1: fd-y21m phage, [U-13C; U-15N], 7 mg

sample_conditions_1: pH: 8; pressure: 1 atm; temperature: 273 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D DARR	sample_1	anisotropic	sample_conditions_1
3D NCACX	sample_1	anisotropic	sample_conditions_1
3D NCOCX	sample_1	anisotropic	sample_conditions_1

Software:

nmrpipe, Delaglio - processing

NMR spectrometers:

Bruker Avance 600 MHz

1	ALA	GLU	GLY	ASP	ASP	PRO	ALA	LYS	ALA	ALA
2	PHE	ASP	SER	LEU	GLN	ALA	SER	ALA	THR	GLU
3	MET	ILE	GLY	TYR	ALA	TRP	ALA	MET	VAL	VAL
4	VAL	ILE	VAL	GLY	ALA	THR	ILE	GLY	ILE	LYS
5	LEU	PHE	LYS	LYS	PHE	THR	SER	LYS	ALA	SER

1	ALA	GLU	GLY	ASP	ASP	PRO	ALA	LYS	ALA	ALA
2	PHE	ASP	SER	LEU	GLN	ALA	SER	ALA	THR	GLU
3	MET	ILE	GLY	TYR	ALA	TRP	ALA	MET	VAL	VAL
4	VAL	ILE	VAL	GLY	ALA	THR	ILE	GLY	ILE	LYS
5	LEU	PHE	LYS	LYS	PHE	THR	SER	LYS	ALA	SER

1	ALA	GLU	GLY	ASP	ASP	PRO	ALA	LYS	ALA	ALA
2	PHE	ASP	SER	LEU	GLN	ALA	SER	ALA	THR	GLU
3	MET	ILE	GLY	TYR	ALA	TRP	ALA	MET	VAL	VAL
4	VAL	ILE	VAL	GLY	ALA	THR	ILE	GLY	ILE	LYS
5	LEU	PHE	LYS	LYS	PHE	THR	SER	LYS	ALA	SER