BMRB Entry 27064
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR27064
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Title: Solution structure of the IgI domain of CD147 PubMed: 28822092
Deposition date: 2017-04-05 Original release date: 2017-12-14
Authors: Jin, Shujuan; Xia, Bin
Citation: Jin, Shujuan; Ding, Pengfei; Chu, Pengxiang; Li, Hongwei; Sun, Jianbo; Liang, Dehai; Song, Fei; Xia, Bin. "Zn(II) can mediate self-association of the extracellular C-terminal domain of CD147." Protein Cell 9, 310-315 (2018).
Assembly members:
CD147, polymer, 107 residues, Formula weight is not available
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET21a(+)
Entity Sequences (FASTA):
CD147: IQLHGPPRVKAVKSSEHINE
GETAMLVCKSESVPPVTDWA
WYKITDSEDKALMNGSESRF
FVSSSQGRSELHIENLNMEA
DPGQYRCNGTSSKGSDQAII
TLRVRSH
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 430 |
| 15N chemical shifts | 113 |
| 1H chemical shifts | 698 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | CD147 | 1 |
Entities:
Entity 1, CD147 107 residues - Formula weight is not available
| 1 | ILE | GLN | LEU | HIS | GLY | PRO | PRO | ARG | VAL | LYS | ||||
| 2 | ALA | VAL | LYS | SER | SER | GLU | HIS | ILE | ASN | GLU | ||||
| 3 | GLY | GLU | THR | ALA | MET | LEU | VAL | CYS | LYS | SER | ||||
| 4 | GLU | SER | VAL | PRO | PRO | VAL | THR | ASP | TRP | ALA | ||||
| 5 | TRP | TYR | LYS | ILE | THR | ASP | SER | GLU | ASP | LYS | ||||
| 6 | ALA | LEU | MET | ASN | GLY | SER | GLU | SER | ARG | PHE | ||||
| 7 | PHE | VAL | SER | SER | SER | GLN | GLY | ARG | SER | GLU | ||||
| 8 | LEU | HIS | ILE | GLU | ASN | LEU | ASN | MET | GLU | ALA | ||||
| 9 | ASP | PRO | GLY | GLN | TYR | ARG | CYS | ASN | GLY | THR | ||||
| 10 | SER | SER | LYS | GLY | SER | ASP | GLN | ALA | ILE | ILE | ||||
| 11 | THR | LEU | ARG | VAL | ARG | SER | HIS |
Samples:
sample_1: CD147, [U-100% 13C; U-100% 15N], 0.5 mM; sodium phosphate 50 mM; sodium chloride 50 mM; EDTA 5 mM
sample_conditions_1: pH: 7.0; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCO | sample_1 | isotropic | sample_conditions_1 |
| 3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
| 3D HCCH-COSY | sample_1 | isotropic | sample_conditions_1 |
| 3D HCCH-TOCSY | sample_1 | isotropic | sample_conditions_1 |
| 3D HBHA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-15N NOESY | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-13C NOESY | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-13C NOESY aromatic | sample_1 | isotropic | sample_conditions_1 |
Software:
AMBER, Case, Darden, Cheatham, III, Simmerling, Wang, Duke, Luo, ... and Kollman - refinement
NMR spectrometers:
- Bruker Avance 500 MHz
- Bruker Avance 800 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts