BMRB Entry 27089
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR27089
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Title: ISCU(D39V) PubMed: 29406711
Deposition date: 2017-04-30 Original release date: 2017-05-23
Authors: Cai, Kai; Markley, John
Citation: Cai, Kai; Frederick, Ronnie; Tonelli, Marco; Markley, John. "ISCU(M108I) and ISCU(D39V) Differ from Wild-Type ISCU in Their Failure To Form Cysteine Desulfurase Complexes Containing Both Frataxin and Ferredoxin" Biochemistry 57, 1491-1500 (2018).
Assembly members:
ISCU, polymer, 135 residues, Formula weight is not available
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET-SUMO
Entity Sequences (FASTA):
ISCU: RLYHKKVVDHYENPRNVGSL
DKTSKNVGTGLVGAPACGVV
MKLQIQVDEKGKIVDARFKT
FGCGSAIASSSLATEWVKGK
TVEEALTIKNTDIAKELCLP
PVKLHCSMLAEDAIKAALAD
YKLKQEPKKGEAEKK
- assigned_chemical_shifts
- spectral_peak_list
| Data type | Count |
| 13C chemical shifts | 211 |
| 15N chemical shifts | 103 |
| 1H chemical shifts | 103 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | ISCU monomer | 1 |
Entities:
Entity 1, ISCU monomer 135 residues - Formula weight is not available
| 1 | ARG | LEU | TYR | HIS | LYS | LYS | VAL | VAL | ASP | HIS | ||||
| 2 | TYR | GLU | ASN | PRO | ARG | ASN | VAL | GLY | SER | LEU | ||||
| 3 | ASP | LYS | THR | SER | LYS | ASN | VAL | GLY | THR | GLY | ||||
| 4 | LEU | VAL | GLY | ALA | PRO | ALA | CYS | GLY | VAL | VAL | ||||
| 5 | MET | LYS | LEU | GLN | ILE | GLN | VAL | ASP | GLU | LYS | ||||
| 6 | GLY | LYS | ILE | VAL | ASP | ALA | ARG | PHE | LYS | THR | ||||
| 7 | PHE | GLY | CYS | GLY | SER | ALA | ILE | ALA | SER | SER | ||||
| 8 | SER | LEU | ALA | THR | GLU | TRP | VAL | LYS | GLY | LYS | ||||
| 9 | THR | VAL | GLU | GLU | ALA | LEU | THR | ILE | LYS | ASN | ||||
| 10 | THR | ASP | ILE | ALA | LYS | GLU | LEU | CYS | LEU | PRO | ||||
| 11 | PRO | VAL | LYS | LEU | HIS | CYS | SER | MET | LEU | ALA | ||||
| 12 | GLU | ASP | ALA | ILE | LYS | ALA | ALA | LEU | ALA | ASP | ||||
| 13 | TYR | LYS | LEU | LYS | GLN | GLU | PRO | LYS | LYS | GLY | ||||
| 14 | GLU | ALA | GLU | LYS | LYS |
Samples:
sample_1: ISCU(D39V), [U-100% 13C; U-100% 15N], 0.5 mM; HEPES 20 mM; sodium chloride 150 mM; TCEP 3 mM; DSS 0.01 mM; EDTA 5 mM
sample_conditions_1: ionic strength: 0.15 M; pH: 7.6; pressure: 1 atm; temperature: 273 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
Software:
TOPSPIN, Bruker Biospin - collection
NMR spectrometers:
- Bruker Avance 600 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts