BMRB Entry 27108
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR27108
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Title: Backbone 1H, 13C, and 15N Chemical Shift Assignments for SH3 domain of Noxa1 PubMed: 28625920
Deposition date: 2017-05-18 Original release date: 2017-09-14
Authors: Shrestha, Pravesh
Citation: Shrestha, Pravesh; Yun, Ji-Hye; Ko, Yoon-Joo; Kim, Myeongkyu; Bae, Yun Soo; Lee, Weontae. "C-terminal tail of NADPH oxidase organizer 1 (Noxo1) mediates interaction with NADPH oxidase activator (Noxa1) in the NOX1 complex" Biochem. Biophys. Res. Commun. 490, 594-600 (2017).
Assembly members:
NOX1_activator, polymer, 64 residues, Formula weight is not available
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET32a
Entity Sequences (FASTA):
NOX1_activator: QRPVLYQVVAQHSYSAQGPE
DLGFRQGDTVDVLCEVDQAW
LEGHCDGRIGIFPKCVVPAG
PRMS
- assigned_chemical_shifts
Data type | Count |
13C chemical shifts | 116 |
15N chemical shifts | 57 |
1H chemical shifts | 55 |
Additional metadata:
Assembly:
Entity Assembly ID | Entity Name | Entity ID |
---|---|---|
1 | NOX1 ativator | 1 |
Entities:
Entity 1, NOX1 ativator 64 residues - Formula weight is not available
1 | GLN | ARG | PRO | VAL | LEU | TYR | GLN | VAL | VAL | ALA | ||||
2 | GLN | HIS | SER | TYR | SER | ALA | GLN | GLY | PRO | GLU | ||||
3 | ASP | LEU | GLY | PHE | ARG | GLN | GLY | ASP | THR | VAL | ||||
4 | ASP | VAL | LEU | CYS | GLU | VAL | ASP | GLN | ALA | TRP | ||||
5 | LEU | GLU | GLY | HIS | CYS | ASP | GLY | ARG | ILE | GLY | ||||
6 | ILE | PHE | PRO | LYS | CYS | VAL | VAL | PRO | ALA | GLY | ||||
7 | PRO | ARG | MET | SER |
Samples:
sample_1: NOX1 ativator, na, mM; HEPES 10 mM; NaCl 100 mM; DTT 1 mM; NaN3 0.01%
sample_conditions_1: pressure: 1 atm; temperature: 298 K
Experiments:
Name | Sample | Sample state | Sample conditions |
---|---|---|---|
2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
Software:
SPARKY, Goddard - chemical shift assignment
NMR spectrometers:
- Bruker DRX 850 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts