BMRB Entry 27325

Name: SH2 domain of Yes1 kinase
Published: 2018-01-16

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR27325

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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title: SH2 domain of Yes1 kinase PubMed: 29343704

Deposition date: 2017-12-03 Original release date: 2018-01-16

Authors: Bachman, Ashleigh; Keramisanou, Dimitra; Kumar M.V., Vasantha; Gelis, Ioannis

Citation: Bachman, Ashleigh; Keramisanou, Dimitra; Xu, Wanping; Beebe, Kristin; Moses, Michael; Vasantha Kumar, M; Gray, Geoffrey; Noor, Radwan Ebna; van der Vaart, Arjan; Neckers, Len; Gelis, Ioannis. "Phosphorylation induced cochaperone unfolding promotes kinase recruitment and client class-specific Hsp90 phosphorylation" Nat. Commun. 9, 265-265 (2018).

Assembly members:
Yes1-SH2, polymer, 110 residues, Formula weight is not available

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pGEX6P1

Entity Sequences (FASTA):
Yes1-SH2: SIQAEEWYFGKMGRKDAERL LLNPGNQRGIFLVRESETTK GAYSLSIRDWDEIRGDNVKH YKIRKLDNGGYYITTRAQFD TLQKLVKHYTEHADGLCHKL TTVCPTVKPQ

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	207
15N chemical shifts	102
1H chemical shifts	102

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Yes1-SH2	1

Entities:

Entity 1, Yes1-SH2 110 residues - Formula weight is not available

1	SER	ILE	GLN	ALA	GLU	GLU	TRP	TYR	PHE	GLY
2	LYS	MET	GLY	ARG	LYS	ASP	ALA	GLU	ARG	LEU
3	LEU	LEU	ASN	PRO	GLY	ASN	GLN	ARG	GLY	ILE
4	PHE	LEU	VAL	ARG	GLU	SER	GLU	THR	THR	LYS
5	GLY	ALA	TYR	SER	LEU	SER	ILE	ARG	ASP	TRP
6	ASP	GLU	ILE	ARG	GLY	ASP	ASN	VAL	LYS	HIS
7	TYR	LYS	ILE	ARG	LYS	LEU	ASP	ASN	GLY	GLY
8	TYR	TYR	ILE	THR	THR	ARG	ALA	GLN	PHE	ASP
9	THR	LEU	GLN	LYS	LEU	VAL	LYS	HIS	TYR	THR
10	GLU	HIS	ALA	ASP	GLY	LEU	CYS	HIS	LYS	LEU
11	THR	THR	VAL	CYS	PRO	THR	VAL	LYS	PRO	GLN

Samples:

sample_2: Yes1-SH2, [U-100% 13C; U-100% 15N], 0.5 mM

sample_1: Yes1-SH2, [U-100% 15N], 0.5 mM

sample_conditions_1: ionic strength: 0.1 M; pH: 7.5; pressure: 1 atm; temperature: 273 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_2	isotropic	sample_conditions_1
3D HNCA	sample_2	isotropic	sample_conditions_1
3D HNCACB	sample_2	isotropic	sample_conditions_1
3D HN(CO)CA	sample_2	isotropic	sample_conditions_1

Software:

SPARKY, Goddard - chemical shift assignment

NMR spectrometers:

Agilent direct drive 800 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts

1	SER	ILE	GLN	ALA	GLU	GLU	TRP	TYR	PHE	GLY
2	LYS	MET	GLY	ARG	LYS	ASP	ALA	GLU	ARG	LEU
3	LEU	LEU	ASN	PRO	GLY	ASN	GLN	ARG	GLY	ILE
4	PHE	LEU	VAL	ARG	GLU	SER	GLU	THR	THR	LYS
5	GLY	ALA	TYR	SER	LEU	SER	ILE	ARG	ASP	TRP
6	ASP	GLU	ILE	ARG	GLY	ASP	ASN	VAL	LYS	HIS
7	TYR	LYS	ILE	ARG	LYS	LEU	ASP	ASN	GLY	GLY
8	TYR	TYR	ILE	THR	THR	ARG	ALA	GLN	PHE	ASP
9	THR	LEU	GLN	LYS	LEU	VAL	LYS	HIS	TYR	THR
10	GLU	HIS	ALA	ASP	GLY	LEU	CYS	HIS	LYS	LEU
11	THR	THR	VAL	CYS	PRO	THR	VAL	LYS	PRO	GLN

1	SER	ILE	GLN	ALA	GLU	GLU	TRP	TYR	PHE	GLY
2	LYS	MET	GLY	ARG	LYS	ASP	ALA	GLU	ARG	LEU
3	LEU	LEU	ASN	PRO	GLY	ASN	GLN	ARG	GLY	ILE
4	PHE	LEU	VAL	ARG	GLU	SER	GLU	THR	THR	LYS
5	GLY	ALA	TYR	SER	LEU	SER	ILE	ARG	ASP	TRP
6	ASP	GLU	ILE	ARG	GLY	ASP	ASN	VAL	LYS	HIS
7	TYR	LYS	ILE	ARG	LYS	LEU	ASP	ASN	GLY	GLY
8	TYR	TYR	ILE	THR	THR	ARG	ALA	GLN	PHE	ASP
9	THR	LEU	GLN	LYS	LEU	VAL	LYS	HIS	TYR	THR
10	GLU	HIS	ALA	ASP	GLY	LEU	CYS	HIS	LYS	LEU
11	THR	THR	VAL	CYS	PRO	THR	VAL	LYS	PRO	GLN

1	SER	ILE	GLN	ALA	GLU	GLU	TRP	TYR	PHE	GLY
2	LYS	MET	GLY	ARG	LYS	ASP	ALA	GLU	ARG	LEU
3	LEU	LEU	ASN	PRO	GLY	ASN	GLN	ARG	GLY	ILE
4	PHE	LEU	VAL	ARG	GLU	SER	GLU	THR	THR	LYS
5	GLY	ALA	TYR	SER	LEU	SER	ILE	ARG	ASP	TRP
6	ASP	GLU	ILE	ARG	GLY	ASP	ASN	VAL	LYS	HIS
7	TYR	LYS	ILE	ARG	LYS	LEU	ASP	ASN	GLY	GLY
8	TYR	TYR	ILE	THR	THR	ARG	ALA	GLN	PHE	ASP
9	THR	LEU	GLN	LYS	LEU	VAL	LYS	HIS	TYR	THR
10	GLU	HIS	ALA	ASP	GLY	LEU	CYS	HIS	LYS	LEU
11	THR	THR	VAL	CYS	PRO	THR	VAL	LYS	PRO	GLN