BMRB Entry 27402
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR27402
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Title: chemical shifts for human Axin DIX domain PubMed: 29561051
Deposition date: 2018-02-14 Original release date: 2020-10-06
Authors: Ward, Meaghan
Citation: Ward, Meaghan; Daniels, Mark; van Kappel, Eline; Maurice, Madelon; Baldus, Marc. "Investigations of dynamic amyloid-like structures of the Wnt signalling pathway by solid-state NMR" Chem. Comm. 54, 3959-3962 (2018).
Assembly members:
human_Axin_DIX_domain, polymer, 96 residues, Formula weight is not available
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: PET24
Entity Sequences (FASTA):
human_Axin_DIX_domain: HHHHHENLYFQQPCDSIVVA
YYFCGEPIPYRTLVRGRAVT
LGQFKELLTKKGSYRYYFKK
VSDEFDCGVVFEEVREDEAV
LPVFEEKIIGKVEKVD
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 273 |
| 15N chemical shifts | 67 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Axin DIX domain, unit 1 | 1 |
| 2 | Axin DIX domain, unit 2 | 1 |
Entities:
Entity 1, Axin DIX domain, unit 1 96 residues - Formula weight is not available
| 1 | HIS | HIS | HIS | HIS | HIS | GLU | ASN | LEU | TYR | PHE | ||||
| 2 | GLN | GLN | PRO | CYS | ASP | SER | ILE | VAL | VAL | ALA | ||||
| 3 | TYR | TYR | PHE | CYS | GLY | GLU | PRO | ILE | PRO | TYR | ||||
| 4 | ARG | THR | LEU | VAL | ARG | GLY | ARG | ALA | VAL | THR | ||||
| 5 | LEU | GLY | GLN | PHE | LYS | GLU | LEU | LEU | THR | LYS | ||||
| 6 | LYS | GLY | SER | TYR | ARG | TYR | TYR | PHE | LYS | LYS | ||||
| 7 | VAL | SER | ASP | GLU | PHE | ASP | CYS | GLY | VAL | VAL | ||||
| 8 | PHE | GLU | GLU | VAL | ARG | GLU | ASP | GLU | ALA | VAL | ||||
| 9 | LEU | PRO | VAL | PHE | GLU | GLU | LYS | ILE | ILE | GLY | ||||
| 10 | LYS | VAL | GLU | LYS | VAL | ASP |
Samples:
sample_1: human Axin DIX domain, [U-100% 13C; U-100% 15N], solid ± 0 mM
sample_conditions_1: ionic strength: 20 mM; pH: 8; pressure: 1 atm; temperature: 278 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 3D NCACX | sample_1 | isotropic | sample_conditions_1 |
| 3D CANCO | sample_1 | isotropic | sample_conditions_1 |
| 3D NCOCX | sample_1 | isotropic | sample_conditions_1 |
| 2D CC Spin Diffusion for seqentials | sample_1 | isotropic | sample_conditions_1 |
Software:
TOPSPIN, Bruker Biospin - collection, processing
NMR spectrometers:
- Bruker Avance 700 MHz
- Bruker Avance 950 MHz