BMRB Entry 27442
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR27442
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Title: Backbone 1H, 13C, and 15N Chemical Shift Assignments for residues 1-136 of yeast Rpn5. PubMed: 30177392
Deposition date: 2018-04-04 Original release date: 2018-09-19
Authors: Zhang, Wenbo; Zhao, Cong; Hu, Yunfei; Jin, Changwen
Citation: Zhang, Wenbo; Zhao, Cong; Hu, Yunfei; Jin, Changwen. "Solution structure of the N-terminal domain of proteasome lid subunit Rpn5" Biochem. Biophys. Res. Commun. 504, 225-230 (2018).
Assembly members:
Rpn5, polymer, 136 residues, 15.6 Da.
Natural source: Common Name: baker's yeast Taxonomy ID: 4932 Superkingdom: Eukaryota Kingdom: Fungi Genus/species: Saccharomyces cerevisiae
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET-28a (+)
Entity Sequences (FASTA):
Rpn5: MSRDAPIKADKDYSQILKEE
FPKIDSLAQNDCNSALDQLL
VLEKKTRQASDLASSKEVLA
KIVDLLASRNKWDDLNEQLT
LLSKKHGQLKLSIQYMIQKV
MEYLKSSKSLDLNTRISVIE
TIRVVTENKIFVEVER
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 557 |
| 15N chemical shifts | 144 |
| 1H chemical shifts | 866 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Rpn5 | 1 |
Entities:
Entity 1, Rpn5 136 residues - 15.6 Da.
| 1 | MET | SER | ARG | ASP | ALA | PRO | ILE | LYS | ALA | ASP | ||||
| 2 | LYS | ASP | TYR | SER | GLN | ILE | LEU | LYS | GLU | GLU | ||||
| 3 | PHE | PRO | LYS | ILE | ASP | SER | LEU | ALA | GLN | ASN | ||||
| 4 | ASP | CYS | ASN | SER | ALA | LEU | ASP | GLN | LEU | LEU | ||||
| 5 | VAL | LEU | GLU | LYS | LYS | THR | ARG | GLN | ALA | SER | ||||
| 6 | ASP | LEU | ALA | SER | SER | LYS | GLU | VAL | LEU | ALA | ||||
| 7 | LYS | ILE | VAL | ASP | LEU | LEU | ALA | SER | ARG | ASN | ||||
| 8 | LYS | TRP | ASP | ASP | LEU | ASN | GLU | GLN | LEU | THR | ||||
| 9 | LEU | LEU | SER | LYS | LYS | HIS | GLY | GLN | LEU | LYS | ||||
| 10 | LEU | SER | ILE | GLN | TYR | MET | ILE | GLN | LYS | VAL | ||||
| 11 | MET | GLU | TYR | LEU | LYS | SER | SER | LYS | SER | LEU | ||||
| 12 | ASP | LEU | ASN | THR | ARG | ILE | SER | VAL | ILE | GLU | ||||
| 13 | THR | ILE | ARG | VAL | VAL | THR | GLU | ASN | LYS | ILE | ||||
| 14 | PHE | VAL | GLU | VAL | GLU | ARG |
Samples:
sample_1: Rpn5, [U-95% 13C; U-95% 15N], 0.3 mM; D2O, [U-99% 2H], 10 % v/v; DSS 0.1 mg/mL; DTT 1 mM; sodium phosphate 50 mM; sodium sulfate 50 mM; trifluoroethanol 2 % v/v; sucrose 5 % w/v
sample_conditions_1: ionic strength: 194 mM; pH: 6.0; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-13C HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCA | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCO | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
| 3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D HN(CO)CA | sample_1 | isotropic | sample_conditions_1 |
| 3D HBHA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-15N TOCSY | sample_1 | isotropic | sample_conditions_1 |
| 3D HCCH-COSY | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-15N NOESY | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-13C NOESY | sample_1 | isotropic | sample_conditions_1 |
Software:
NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing
NMRView, Johnson, One Moon Scientific - chemical shift assignment, data analysis, peak picking
NMR spectrometers:
- Bruker Avance 500 MHz
- Bruker Avance 950 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts