BMRB Entry 27503

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PDB ID: 6rk3
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR27503
MolProbity Validation Chart

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Title:
Backbone and side chain NMR assignments for the ribosome Elongation Factor P (EfP) from Staphylococcus aureus
Deposition date:
2018-06-04
Original release date:
2018-10-03
Authors:
Golubev, Alexander; Validov, Shamil; Klochkov, Vladimir; Aganov, Albert; Khusainov, Iskander; Yusupov, Marat
Citation:

Citation: Usachev, Konstantin; Golubev, Alexander; Validov, Shamil; Klochkov, Vladimir; Aganov, Albert; Khusainov, Iskander; Yusupov, Marat. "Backbone and side chain NMR assignments for the ribosome Elongation Factor P (EF-P) from Staphylococcus aureus"  Biomol. NMR Assignments 12, 351-355 (2018).
PubMed: 30099718

Assembly members:

Assembly members:
Ef-P, polymer, 185 residues, 20545.9478 Da.

Natural source:

Natural source:   Common Name: Staphylococcus aureus   Taxonomy ID: 1280   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Staphylococcus aureus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET28a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Ef-P: MISVNDFKTGLTISVDNAIW KVIDFQHVKPGKGSAFVRSK LRNLRTGAIQEKTFRAGEKV EPAMIENRRMQYLYADGDNH VFMDNESFEQTELSSDYLKE ELNYLKEGMEVQIQTYEGET IGVELPKTVELTVTETEPGI KGDTATGATKSATVETGYTL NVPLFVNEGDVLIINTGDGS YISRG

Data sets:
Data typeCount
13C chemical shifts556
15N chemical shifts152
1H chemical shifts674

Additional metadata:

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Assembly:

Entity Assembly IDEntity NameEntity ID
1Ef-P1

Entities:

Entity 1, Ef-P 185 residues - 20545.9478 Da.

1   METILESERVALASNASPPHELYSTHRGLY
2   LEUTHRILESERVALASPASNALAILETRP
3   LYSVALILEASPPHEGLNHISVALLYSPRO
4   GLYLYSGLYSERALAPHEVALARGSERLYS
5   LEUARGASNLEUARGTHRGLYALAILEGLN
6   GLULYSTHRPHEARGALAGLYGLULYSVAL
7   GLUPROALAMETILEGLUASNARGARGMET
8   GLNTYRLEUTYRALAASPGLYASPASNHIS
9   VALPHEMETASPASNGLUSERPHEGLUGLN
10   THRGLULEUSERSERASPTYRLEULYSGLU
11   GLULEUASNTYRLEULYSGLUGLYMETGLU
12   VALGLNILEGLNTHRTYRGLUGLYGLUTHR
13   ILEGLYVALGLULEUPROLYSTHRVALGLU
14   LEUTHRVALTHRGLUTHRGLUPROGLYILE
15   LYSGLYASPTHRALATHRGLYALATHRLYS
16   SERALATHRVALGLUTHRGLYTYRTHRLEU
17   ASNVALPROLEUPHEVALASNGLUGLYASP
18   VALLEUILEILEASNTHRGLYASPGLYSER
19   TYRILESERARGGLY

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks