BMRB Entry 27555

Name: Backbone assignment of Rip2Card
Published: 2018-11-15

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR27555

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Backbone assignment of Rip2Card

Deposition date:

2018-07-24

Original release date:

2018-11-15

Authors:

Wallmann, Arndt; Oschkinat, Hartmut

Citation:

Citation: Pellegrini, Erika; Desfosses, Ambroise; Wallmann, Arndt; Schulze, Wiebke; Rehbein, Kristina; Mas, Philippe; Signor, Luca; Gaudon, Stephanie; Zenkeviciute, Grasilda; Malet, Helene; Gutsche, Irina; Sachse, Carsten; Schoehn, Guy; Oschkinat, Hartmut; Cusack, Stephen. "RIP2 filament formation is required for NOD2 dependent NF-B signalling" Nat. Commun. 9, 4043-4043 (2018).
PubMed: 30279485

Assembly members:

Assembly members:
Rip2Card, polymer, 107 residues, 12248.0952 Da.

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pETM40

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Rip2Card: AIAQQWIQSKREDIVNQMTE ACLNQSLDALLSRDLIMKED YELVSTKPTRTSKVRQLLDT TDIQGEEFAKVIVQKLKDNK QMGLQPYPEILVVSRSPSLN LLQNKSM

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list

Data type	Count
13C chemical shifts	185
15N chemical shifts	63
1H chemical shifts	63

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	CARD domain	1

Entities:

Entity 1, CARD domain 107 residues - 12248.0952 Da.

1

ALA

ILE

ALA

GLN

TRP

ILE

GLN

SER

LYS

2

ARG

GLU

ASP

ILE

VAL

ASN

GLN

MET

THR

GLU

3

ALA

CYS

LEU

ASN

GLN

SER

LEU

ASP

ALA

LEU

4

LEU

SER

ARG

ASP

LEU

ILE

MET

LYS

GLU

ASP

5

TYR

GLU

LEU

VAL

SER

THR

LYS

PRO

THR

ARG

6

THR

SER

LYS

VAL

ARG

GLN

LEU

ASP

THR

7

THR

ASP

ILE

GLN

GLY

GLU

PHE

ALA

LYS

8

VAL

ILE

VAL

GLN

LYS

LEU

LYS

ASP

ASN

LYS

9

GLN

MET

GLY

LEU

GLN

PRO

TYR

PRO

GLU

ILE

10

LEU

VAL

SER

ARG

SER

PRO

SER

LEU

ASN

11

LEU

GLN

ASN

LYS

SER

MET

Samples:

sample_1: Rip2Card, [U-100% 13C; U-100% 15N; 100% D2O], 2 mg

sample_conditions_1: pH: 8; temperature: 295 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D (H)CANH	sample_1	isotropic	sample_conditions_1
3D (HCO)CA(CO)NH	sample_1	isotropic	sample_conditions_1
3D (HCA)CB(CA)NH	sample_1	isotropic	sample_conditions_1
3D (HCA)CB(CACO)NH	sample_1	isotropic	sample_conditions_1
3D (H)CONH	sample_1	isotropic	sample_conditions_1
3D (H)CO(CA)NH	sample_1	isotropic	sample_conditions_1

Software:

CcpNmr_Analysis v2.4, CCPN - chemical shift assignment, collection, processing

Bruker Biospin, Bruker Biospin - chemical shift assignment, collection, processing

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMR spectrometers:

Bruker Avance 800 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks