BMRB Entry 27679

Title:
1H/13C/15N Assignments for the TM domains of the KcsA potassium channel
Deposition date:
2018-11-05
Original release date:
2019-11-05
Authors:
Medeiros-Silva, Joao; Jekhmane, Shehrazade; Li, Jing; Kummerer, Felix; Muller-Hermes, Christoph; Baldus, Marc; Roux, Benoit; Weingarth, Markus
Citation:

Citation: Jekhmane, Shehrazade; Medeiros-Silva, Joao; Li, Jing; Kummerer, Felix; Muller-Hermes, Christoph; Baldus, Marc; Roux, Benoit; Weingarth, Markus. "Shifts in the selectivity filter dynamics cause modal gating in K+ channels"  Nat. Commun. 10, 123-123 (2019).
PubMed: 30631074

Assembly members:

Assembly members:
KcsA_E71I, polymer, 166 residues, 17694 Da.

Natural source:

Natural source:   Common Name: Streptomyces lividans   Taxonomy ID: 1916   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Streptomyces lividans

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: PQE60

Data sets:
Data typeCount
13C chemical shifts79
15N chemical shifts34
1H chemical shifts34

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1KcsA Monomer E71I, 11
2KcsA Monomer E71I, 21
3KcsA Monomer E71I, 31
4KcsA Monomer E71I, 41

Entities:

Entity 1, KcsA Monomer E71I, 1 166 residues - 17694 Da.

Residues 1-27; 51-61; 82-87; 112-160 are in contact with the cytoplasmic/extracellular environment Residues 28-50; 62-81;88-111 are embedded in the membrane Residues 74-80 form the filter Residues 161-166 form the His-Tag

1   METPROPROMETLEUSERGLYLEULEUALA
2   ARGLEUVALLYSLEULEULEUGLYARGHIS
3   GLYSERALALEUHISTRPARGALAALAGLY
4   ALAALATHRVALLEULEUVALILEVALLEU
5   LEUALAGLYSERTYRLEUALAVALLEUALA
6   GLUARGGLYALAPROGLYALAGLNLEUILE
7   THRTYRPROARGALALEUTRPTRPSERVAL
8   ILETHRALATHRTHRVALGLYTYRGLYASP
9   LEUTYRPROVALTHRLEUTRPGLYARGLEU
10   VALALAVALVALVALMETVALALAGLYILE
11   THRSERPHEGLYLEUVALTHRALAALALEU
12   ALATHRTRPPHEVALGLYARGGLUGLNGLU
13   ARGARGGLYHISPHEVALARGHISSERGLU
14   LYSALAALAGLUGLUALATYRTHRARGTHR
15   THRARGALALEUHISGLUARGPHEASPARG
16   LEUGLUARGMETLEUASPASPASNARGARG
17   HISHISHISHISHISHIS

Related Database Links:

UNP P0A334
EMBL 1f6g
NCBI 1916
PIR S60172
PDB 1BL8
AlphaFold Q54397

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks