BMRB Entry 27705
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR27705
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Title: Backbone 1H, 13C, and 15N Chemical Shift Assignments for Guanylate Cyclase Activating Protein-5 (GCAP5) in Zebrafish Photoreceptors PubMed: 30559291
Deposition date: 2018-11-26 Original release date: 2019-01-08
Authors: Cudia, Diana; Ames, James
Citation: Peshenko, Igor; Yu, Qinhong; Lim, Sunghyuk; Cudia, Diana; Dizhoor, Alexander; Ames, James. "Retinal degeneration 3 (RD3) protein, a retinal guanylyl cyclase regulator, forms a monomeric and elongated four helix bundle" J. Biol. Chem. 94, 2318-2328 (2019).
Assembly members:
GCAP5, polymer, 198 residues, Formula weight is not available
Natural source: Common Name: E. coli Taxonomy ID: 562 Superkingdom: Bacteria Kingdom: not available Genus/species: Escherichia coli
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET21
Entity Sequences (FASTA):
GCAP5: MGDSSSMSATELSACKCHQW
YRKFMTECPSGQLTFYEFKK
FFGLKNLSEKSNAYVNTMFK
TFDIDDDGCIDFMEYVAALS
LVLKGGVQQKLRWYFKLFDM
DGSGCIDKDELLLIFKAVQA
INGAEPEISAEDLADIVFNK
IDVNGDGELSLEEFMEGISA
DEKISEMLTQSLDLTRIVSN
IYNDSYIEQEAEIIEDQA
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 579 |
| 15N chemical shifts | 160 |
| 1H chemical shifts | 812 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | GCAP5 | 1 |
Entities:
Entity 1, GCAP5 198 residues - Formula weight is not available
| 1 | MET | GLY | ASP | SER | SER | SER | MET | SER | ALA | THR | ||||
| 2 | GLU | LEU | SER | ALA | CYS | LYS | CYS | HIS | GLN | TRP | ||||
| 3 | TYR | ARG | LYS | PHE | MET | THR | GLU | CYS | PRO | SER | ||||
| 4 | GLY | GLN | LEU | THR | PHE | TYR | GLU | PHE | LYS | LYS | ||||
| 5 | PHE | PHE | GLY | LEU | LYS | ASN | LEU | SER | GLU | LYS | ||||
| 6 | SER | ASN | ALA | TYR | VAL | ASN | THR | MET | PHE | LYS | ||||
| 7 | THR | PHE | ASP | ILE | ASP | ASP | ASP | GLY | CYS | ILE | ||||
| 8 | ASP | PHE | MET | GLU | TYR | VAL | ALA | ALA | LEU | SER | ||||
| 9 | LEU | VAL | LEU | LYS | GLY | GLY | VAL | GLN | GLN | LYS | ||||
| 10 | LEU | ARG | TRP | TYR | PHE | LYS | LEU | PHE | ASP | MET | ||||
| 11 | ASP | GLY | SER | GLY | CYS | ILE | ASP | LYS | ASP | GLU | ||||
| 12 | LEU | LEU | LEU | ILE | PHE | LYS | ALA | VAL | GLN | ALA | ||||
| 13 | ILE | ASN | GLY | ALA | GLU | PRO | GLU | ILE | SER | ALA | ||||
| 14 | GLU | ASP | LEU | ALA | ASP | ILE | VAL | PHE | ASN | LYS | ||||
| 15 | ILE | ASP | VAL | ASN | GLY | ASP | GLY | GLU | LEU | SER | ||||
| 16 | LEU | GLU | GLU | PHE | MET | GLU | GLY | ILE | SER | ALA | ||||
| 17 | ASP | GLU | LYS | ILE | SER | GLU | MET | LEU | THR | GLN | ||||
| 18 | SER | LEU | ASP | LEU | THR | ARG | ILE | VAL | SER | ASN | ||||
| 19 | ILE | TYR | ASN | ASP | SER | TYR | ILE | GLU | GLN | GLU | ||||
| 20 | ALA | GLU | ILE | ILE | GLU | ASP | GLN | ALA |
Samples:
GCAP5_sample: GCAP5, [U-100% 13C; U-100% 15N], 500 mM; magnesium chloride, [U-100% 13C; U-100% 15N], 5 mM; MES 5 mM; DTT, [U-99% 2H], 3.5 mM; sodium azide 0.04 % w/v; D2O, [U-99% 2H], 7.5%; H2O 92.5%
sample_conditions_1: pH: 6.5; temperature: 303 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | GCAP5_sample | isotropic | sample_conditions_1 |
| 3D HNCO | GCAP5_sample | isotropic | sample_conditions_1 |
| 3D HNCACB | GCAP5_sample | isotropic | sample_conditions_1 |
| 3D CBCA(CO)NH | GCAP5_sample | isotropic | sample_conditions_1 |
| 3D HBHA(CO)NH | GCAP5_sample | isotropic | sample_conditions_1 |
| 3D HNCA | GCAP5_sample | isotropic | sample_conditions_1 |
| 3D HN(CO)CA | GCAP5_sample | isotropic | sample_conditions_1 |
Software:
NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing
NMRDraw, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing, refinement
SPARKY, Goddard - chemical shift assignment, data analysis, peak picking
NMR spectrometers:
- Bruker Avance 800 MHz
- Bruker Avance 600 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts