BMRB Entry 27767
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR27767
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Title: Backbone 1H, 13C, and 15N Chemical Shift Assignments for bacteriophage protein Gp46 PubMed: 30830594
Deposition date: 2019-01-26 Original release date: 2019-03-08
Authors: Liu, Bing
Citation: Zhang, Peipei; Wang, Zhihao; Zhao, Siyu; Wang, Yawen; Matthews, Steve; Liu, Bing. "1H, 13C and 15N NMR assignments of Bacillus subtilis bacteriophage SPO1 protein Gp46" Biomol. NMR Assignments 13, 245-247 (2019).
Assembly members:
Gp46, polymer, 77 residues, Formula weight is not available
Natural source: Common Name: E. coli Taxonomy ID: 562 Superkingdom: Bacteria Kingdom: not available Genus/species: Escherichia coli
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET-SUMO
Entity Sequences (FASTA):
Gp46: MMTEDQKFKYLTKIEELEAG
CFSDWTKEDITGDLKYLKKG
IIEESIELIRAVNGLTYSEE
LHDFTQEIIEELDISPL
- assigned_chemical_shifts
Data type | Count |
13C chemical shifts | 355 |
15N chemical shifts | 76 |
1H chemical shifts | 558 |
Additional metadata:
Assembly:
Entity Assembly ID | Entity Name | Entity ID |
---|---|---|
1 | Gp46 | 1 |
Entities:
Entity 1, Gp46 77 residues - Formula weight is not available
1 | MET | MET | THR | GLU | ASP | GLN | LYS | PHE | LYS | TYR | ||||
2 | LEU | THR | LYS | ILE | GLU | GLU | LEU | GLU | ALA | GLY | ||||
3 | CYS | PHE | SER | ASP | TRP | THR | LYS | GLU | ASP | ILE | ||||
4 | THR | GLY | ASP | LEU | LYS | TYR | LEU | LYS | LYS | GLY | ||||
5 | ILE | ILE | GLU | GLU | SER | ILE | GLU | LEU | ILE | ARG | ||||
6 | ALA | VAL | ASN | GLY | LEU | THR | TYR | SER | GLU | GLU | ||||
7 | LEU | HIS | ASP | PHE | THR | GLN | GLU | ILE | ILE | GLU | ||||
8 | GLU | LEU | ASP | ILE | SER | PRO | LEU |
Samples:
sample_1: Gp46, [U-100% 13C; U-100% 15N], 0.5 mM; sodium chloride 300 mM; TCEP 1 mM; potassium phosphate 50 mM
sample_conditions_1: ionic strength: 0.35 M; pH: 6.5; pressure: 1 atm; temperature: 300 K
Experiments:
Name | Sample | Sample state | Sample conditions |
---|---|---|---|
2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
3D HNCO | sample_1 | isotropic | sample_conditions_1 |
3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
3D HN(CA)CO | sample_1 | isotropic | sample_conditions_1 |
3D HCCH-TOCSY | sample_1 | isotropic | sample_conditions_1 |
3D 1H-15N NOESY | sample_1 | isotropic | sample_conditions_1 |
3D 1H-13C NOESY aliphatic | sample_1 | isotropic | sample_conditions_1 |
2D 1H-13C HSQC aromatic | sample_1 | isotropic | sample_conditions_1 |
3D HBHA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
3D (H)CCH-TOCSY | sample_1 | isotropic | sample_conditions_1 |
Software:
NMRView, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax, Johnson, One Moon Scientific - chemical shift assignment, processing
NMR spectrometers:
- Bruker Avance 600 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts