BMRB Entry 27924
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR27924
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Title: scAtg8(K26P,C33V,G116C) PubMed: 31399562
Deposition date: 2019-05-23 Original release date: 2019-08-06
Authors: Royappa, Grace; Zheng, Yumei; Yu, Qiu; Schulman, Brenda
Citation: Zheng, Yumei; Qiu, Yu; Grace, Christy; Liu, Xu; Klionsky, Daniel; Schulman, Brenda. "A switch element in the autophagy E2 Atg3 mediates allosteric regulation across the lipidation cascade" Nat. Commun. 10, 3600-3600 (2019).
Assembly members:
scAtg8, polymer, 118 residues, Formula weight is not available
Natural source: Common Name: baker's yeast Taxonomy ID: 4932 Superkingdom: Eukaryota Kingdom: Fungi Genus/species: Saccharomyces cerevisiae
Experimental source: Production method: recombinant technology Host organism: Saccharomyces cerevisiae Vector: pGEX-4T-1
Entity Sequences (FASTA):
scAtg8: GSMKSTFKSEYPFEKRKAES
ERIADRFPNRIPVIVEKAEK
SDIPEIDKRKYLVPADLTVG
QFVYVIRKRIMLPPEKAIFI
FVNDTLPPTAALMSAIYQEH
KDKDGFLYVTYSGENTFC
- assigned_chemical_shifts
Data type | Count |
13C chemical shifts | 192 |
15N chemical shifts | 100 |
1H chemical shifts | 100 |
Additional metadata:
Assembly:
Entity Assembly ID | Entity Name | Entity ID |
---|---|---|
1 | scAtg8 | 1 |
Entities:
Entity 1, scAtg8 118 residues - Formula weight is not available
1 | GLY | SER | MET | LYS | SER | THR | PHE | LYS | SER | GLU | ||||
2 | TYR | PRO | PHE | GLU | LYS | ARG | LYS | ALA | GLU | SER | ||||
3 | GLU | ARG | ILE | ALA | ASP | ARG | PHE | PRO | ASN | ARG | ||||
4 | ILE | PRO | VAL | ILE | VAL | GLU | LYS | ALA | GLU | LYS | ||||
5 | SER | ASP | ILE | PRO | GLU | ILE | ASP | LYS | ARG | LYS | ||||
6 | TYR | LEU | VAL | PRO | ALA | ASP | LEU | THR | VAL | GLY | ||||
7 | GLN | PHE | VAL | TYR | VAL | ILE | ARG | LYS | ARG | ILE | ||||
8 | MET | LEU | PRO | PRO | GLU | LYS | ALA | ILE | PHE | ILE | ||||
9 | PHE | VAL | ASN | ASP | THR | LEU | PRO | PRO | THR | ALA | ||||
10 | ALA | LEU | MET | SER | ALA | ILE | TYR | GLN | GLU | HIS | ||||
11 | LYS | ASP | LYS | ASP | GLY | PHE | LEU | TYR | VAL | THR | ||||
12 | TYR | SER | GLY | GLU | ASN | THR | PHE | CYS |
Samples:
sample_1: scAtg8, [U-100% 13C; U-100% 15N], 0.4 mM; sodium chloride 100 mM; MES 20 mM
sample_conditions_1: ionic strength: 100 mM; pH: 6.5; pressure: 1 atm; temperature: 298 K
Experiments:
Name | Sample | Sample state | Sample conditions |
---|---|---|---|
2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
3D HNCA | sample_1 | isotropic | sample_conditions_1 |
3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
3D HNCO | sample_1 | isotropic | sample_conditions_1 |
3D HN(CO)CA | sample_1 | isotropic | sample_conditions_1 |
3D 1H-15N NOESY | sample_1 | isotropic | sample_conditions_1 |
Software:
CARA, Keller and Wuthrich - chemical shift assignment, data analysis
TOPSPIN, Bruker Biospin - collection, processing
NMR spectrometers:
- Bruker Avance 600 MHz
- Bruker Avance 800 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts