BMRB Entry 27980
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR27980
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Title: 13C-chemical-shifts-for_SPA_amyloid-beta42_oligomer PubMed: 31771980
Deposition date: 2019-07-22 Original release date: 2019-11-21
Authors: Ishii, Yoshitaka
Citation: Xiao, Yiling; Matsuda, Isamu; Inoue, Masafumi; Sasahara, Tomoya; Hoshi, Minako; Ishii, Yoshitaka. "NMR-based site-resolved profiling of beta-amyloid misfolding: structural transition from pathologically relevant spherical oligomer to fibril" J. Biol. Chem. 295, 458-467 (2020).
Assembly members:
SPA-amyloid_beta_oligomer, polymer, 42 residues, Formula weight is not available
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: chemical synthesis
Entity Sequences (FASTA):
SPA-amyloid_beta_oligomer: DAEFRHDSGYEVHHQKLVFF
AEDVGSNKGAIIGLMVGGVV
IA
- assigned_chemical_shifts
Data type | Count |
13C chemical shifts | 74 |
Additional metadata:
Assembly:
Entity Assembly ID | Entity Name | Entity ID |
---|---|---|
1 | 42-residue amyloid-beta | 1 |
Entities:
Entity 1, 42-residue amyloid-beta 42 residues - Formula weight is not available
1 | ASP | ALA | GLU | PHE | ARG | HIS | ASP | SER | GLY | TYR | ||||
2 | GLU | VAL | HIS | HIS | GLN | LYS | LEU | VAL | PHE | PHE | ||||
3 | ALA | GLU | ASP | VAL | GLY | SER | ASN | LYS | GLY | ALA | ||||
4 | ILE | ILE | GLY | LEU | MET | VAL | GLY | GLY | VAL | VAL | ||||
5 | ILE | ALA |
Samples:
sample_1: SPA-amyloid_beta_oligomer, [U-98% 13C; U-98% 15N], 20 % w/w
sample_conditions_1: pressure: 1 atm; temperature: 293 K
Experiments:
Name | Sample | Sample state | Sample conditions |
---|---|---|---|
2D 13C-13C correlation MAS | sample_1 | isotropic | sample_conditions_1 |
Software:
NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing
NMR spectrometers:
- Bruker Avance 400 MHz