BMRB Entry 28028

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PDB ID: 6xxu
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full
BMRB Entry DOI: doi:10.13018/BMR28028
MolProbity Validation Chart

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Title:
1H, 13C, 15N backbone and side-chain assignment of the native form of UbcH7 (UBE2L3)
Deposition date:
2019-10-09
Original release date:
2020-01-30
Authors:
Marousis, Konstantinos; Seliami, Anastasia; Birkou, Maria; Spyroulias, Georgios
Citation:

Citation: Marousis, Konstantinos; Birkou, Maria; Asimakopoulou, Antonia; Spyroulias, Georgios. "1H, 13C, 15N backbone and side-chain resonance assignment of the native form of UbcH7 (UBE2L3) through solution NMR spectroscopy"  Biomol. NMR Assignments 14, 73-78 (2020).
PubMed: 31792831

Assembly members:

Assembly members:
UbcH7, polymer, 154 residues, 17861.58 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pGEX-4T-1

Entity Sequences (FASTA):

Entity Sequences (FASTA):
UbcH7: MAASRRLMKELEEIRKCGMK NFRNIQVDEANLLTWQGLIV PDNPPYDKGAFRIEINFPAE YPFKPPKITFKTKIYHPNID EKGQVCLPVISAENWKPATK TDQVIQSLIALVNDPQPEHP LRADLAEEYSKDRKKFCKNA EEFTKKYGEKRPVD

Data sets:
Data typeCount
13C chemical shifts684
15N chemical shifts157
1H chemical shifts1116

Additional metadata:

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Assembly:

Entity Assembly IDEntity NameEntity ID
1UbcH71

Entities:

Entity 1, UbcH7 154 residues - 17861.58 Da.

1   METALAALASERARGARGLEUMETLYSGLU
2   LEUGLUGLUILEARGLYSCYSGLYMETLYS
3   ASNPHEARGASNILEGLNVALASPGLUALA
4   ASNLEULEUTHRTRPGLNGLYLEUILEVAL
5   PROASPASNPROPROTYRASPLYSGLYALA
6   PHEARGILEGLUILEASNPHEPROALAGLU
7   TYRPROPHELYSPROPROLYSILETHRPHE
8   LYSTHRLYSILETYRHISPROASNILEASP
9   GLULYSGLYGLNVALCYSLEUPROVALILE
10   SERALAGLUASNTRPLYSPROALATHRLYS
11   THRASPGLNVALILEGLNSERLEUILEALA
12   LEUVALASNASPPROGLNPROGLUHISPRO
13   LEUARGALAASPLEUALAGLUGLUTYRSER
14   LYSASPARGLYSLYSPHECYSLYSASNALA
15   GLUGLUPHETHRLYSLYSTYRGLYGLULYS
16   ARGPROVALASP

Related Database Links:

UNP P68036
AlphaFold Q9HAV1

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks