BMRB Entry 28029
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR28029
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry
Title: The short fibrillation segment in FUS-LC domain PubMed: 31805282
Deposition date: 2019-10-11 Original release date: 2021-03-11
Authors: Qiang, Wei
Citation: Ding, Xiufang; Sun, Fude; Chen, Jialin; Chen, Long; Tobin-Miyaji, Yuto; Xue, Song; Qiang, Wei; Luo, Shi-Zhong. "Amyloid-Forming Segment Induces Aggregation of FUS-LC Domain from Phase Separation Modulated by Site-Specific Phosphorylation" J. Mol. Biol. 432, 467-483 (2020).
Assembly members:
FUS_segment, polymer, 16 residues, Formula weight is not available
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: chemical synthesis Host organism: na Vector: na
Entity Sequences (FASTA):
FUS_segment: YGQSSYSSYGQSQNTG
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 68 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | FUS segment | 1 |
Entities:
Entity 1, FUS segment 16 residues - Formula weight is not available
| 1 | TYR | GLY | GLN | SER | SER | TYR | SER | SER | TYR | GLY | ||||
| 2 | GLN | SER | GLN | ASN | THR | GLY |
Samples:
sample_1: FUS segment, [U-13C], 50 uM
sample_conditions_1: ionic strength: 10 mM; pH: 7.4; pressure: 1 atm; temperature: 280 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 13C/13C spin diffusion | sample_1 | isotropic | sample_conditions_1 |
Software:
X-PLOR_NIH, Schwieters, Kuszewski, Tjandra and Clore - structure solution
NMR spectrometers:
- Bruker INOVA 600 MHz