BMRB Entry 30043

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PDB ID: 5iq5
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR30043
MolProbity Validation Chart

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Title:
NMR solution structure of Mayaro virus macro domain
Deposition date:
2016-03-10
Original release date:
2017-12-13
Authors:
Melekis, E.; Tsika, A.; Bentrop, D.; Papageorgiou, N.; Coutard, B.; Spyroulias, G.
Citation:

Citation: Tsika, A.; Melekis, E.; Tsatsouli, S.; Papageorgiou, N.; Mate, M.; Canard, B.; Coutard, B.; Bentrop, D.; Spyroulias, G.. "Deciphering the Nucleotide and RNA Binding Selectivity of the Mayaro Virus Macro Domain"  J. Mol. Biol. 431, 2283-2297 (2019).
PubMed: 30998933

Assembly members:

Assembly members:
entity_1, polymer, 166 residues, 18147.494 Da.

Natural source:

Natural source:   Common Name: MAYV   Taxonomy ID: 374990   Superkingdom: Viruses   Kingdom: not available   Genus/species: Alphavirus Mayaro virus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli BL21(DE3)   Vector: pDest14

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: MAPAYTVKRADIATAIEDAV VNAANHRGQVGDGVCRAVAR KWPQAFRNAATPVGTAKTVK CDETYIIHAVGPNFNNTSEA EGDRDLAAAYRAVAAEINRL SISSVAIPLLSTGIFSAGKD RVHQSLSHLLAAMDTTEARV TIYCRDKTWEQKIKTVLQNR HHHHHH

Data sets:
Data typeCount
13C chemical shifts441
15N chemical shifts136
1H chemical shifts917

Additional metadata:

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Assembly:

Entity Assembly IDEntity NameEntity ID
1entity_11

Entities:

Entity 1, entity_1 166 residues - 18147.494 Da.

1   METALAPROALATYRTHRVALLYSARGALA
2   ASPILEALATHRALAILEGLUASPALAVAL
3   VALASNALAALAASNHISARGGLYGLNVAL
4   GLYASPGLYVALCYSARGALAVALALAARG
5   LYSTRPPROGLNALAPHEARGASNALAALA
6   THRPROVALGLYTHRALALYSTHRVALLYS
7   CYSASPGLUTHRTYRILEILEHISALAVAL
8   GLYPROASNPHEASNASNTHRSERGLUALA
9   GLUGLYASPARGASPLEUALAALAALATYR
10   ARGALAVALALAALAGLUILEASNARGLEU
11   SERILESERSERVALALAILEPROLEULEU
12   SERTHRGLYILEPHESERALAGLYLYSASP
13   ARGVALHISGLNSERLEUSERHISLEULEU
14   ALAALAMETASPTHRTHRGLUALAARGVAL
15   THRILETYRCYSARGASPLYSTHRTRPGLU
16   GLNLYSILELYSTHRVALLEUGLNASNARG
17   HISHISHISHISHISHIS

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