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PDB ID: 5j8h
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR30063
MolProbity Validation Chart
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
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Citation: Lee, K.; Alphonse, S.; Piserchio, A.; Tavares, C.; Giles, D.; Wellmann, R.; Dalby, K.; Ghose, R.. "Structural Basis for the Recognition of Eukaryotic Elongation Factor 2 Kinase by Calmodulin" Structure 24, 1441-1451 (2016).
PubMed: 27499441
Assembly members:
Calmodulin, polymer, 148 residues, 16721.350 Da.
Eukaryotic elongation factor 2 kinase, polymer, 27 residues, 3167.618 Da.
CALCIUM ION, non-polymer, 40.078 Da.
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET28b
Entity Sequences (FASTA):
Calmodulin: ADQLTEEQIAEFKEAFSLFD
KDGDGTITTKELGTVMRSLG
QNPTEAELQDMINEVDADGN
GTIDFPEFLTMMARKMKDTD
SEEEIREAFRVFDKDGNGYI
SAAELRHVMTNLGEKLTDEE
VDEMIREADIDGDGQVNYEE
FVQMMTAK
Eukaryotic elongation factor 2 kinase: SPANSFHFKEAWKHAIQKAK
HMPDPWA
Data type | Count |
13C chemical shifts | 737 |
15N chemical shifts | 183 |
1H chemical shifts | 1125 |
Entity Assembly ID | Entity Name | Entity ID |
---|---|---|
1 | entity_1 | 1 |
2 | entity_2 | 2 |
3 | CALCIUM ION | 3 |
4 | CALCIUM ION | 3 |
Entity 1, entity_1 148 residues - 16721.350 Da.
1 | ALA | ASP | GLN | LEU | THR | GLU | GLU | GLN | ILE | ALA | ||||
2 | GLU | PHE | LYS | GLU | ALA | PHE | SER | LEU | PHE | ASP | ||||
3 | LYS | ASP | GLY | ASP | GLY | THR | ILE | THR | THR | LYS | ||||
4 | GLU | LEU | GLY | THR | VAL | MET | ARG | SER | LEU | GLY | ||||
5 | GLN | ASN | PRO | THR | GLU | ALA | GLU | LEU | GLN | ASP | ||||
6 | MET | ILE | ASN | GLU | VAL | ASP | ALA | ASP | GLY | ASN | ||||
7 | GLY | THR | ILE | ASP | PHE | PRO | GLU | PHE | LEU | THR | ||||
8 | MET | MET | ALA | ARG | LYS | MET | LYS | ASP | THR | ASP | ||||
9 | SER | GLU | GLU | GLU | ILE | ARG | GLU | ALA | PHE | ARG | ||||
10 | VAL | PHE | ASP | LYS | ASP | GLY | ASN | GLY | TYR | ILE | ||||
11 | SER | ALA | ALA | GLU | LEU | ARG | HIS | VAL | MET | THR | ||||
12 | ASN | LEU | GLY | GLU | LYS | LEU | THR | ASP | GLU | GLU | ||||
13 | VAL | ASP | GLU | MET | ILE | ARG | GLU | ALA | ASP | ILE | ||||
14 | ASP | GLY | ASP | GLY | GLN | VAL | ASN | TYR | GLU | GLU | ||||
15 | PHE | VAL | GLN | MET | MET | THR | ALA | LYS |
Entity 2, entity_2 27 residues - 3167.618 Da.
1 | SER | PRO | ALA | ASN | SER | PHE | HIS | PHE | LYS | GLU | ||||
2 | ALA | TRP | LYS | HIS | ALA | ILE | GLN | LYS | ALA | LYS | ||||
3 | HIS | MET | PRO | ASP | PRO | TRP | ALA |
Entity 3, CALCIUM ION - 40.078 Da.
1 | CA |
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SPARKY: Backbone
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