BMRB Entry 30076

Name: Domain 4 Segment 6 of voltage-gated sodium channel Nav1.4
Published: 2017-05-15

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PDB ID: 5jr0
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR30076
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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title: Domain 4 Segment 6 of voltage-gated sodium channel Nav1.4 PubMed: 30389410

Deposition date: 2016-05-05 Original release date: 2017-05-15

Authors: Niitsu, Ai; Egawa, Ayako; Ikeda, Keisuke; Tachibana, Kazuo; Fujiwara, Toshimichi

Citation: Niitsu, Ai; Egawa, Ayako; Ikeda, Keisuke; Tachibana, Kazuo; Fujiwara, Toshimichi. "Veratridine binding to a transmembrane helix of sodium channel Na" Bioorg. Med. Chem. 26, 5644-5653 (2018).

Assembly members:
Sodium channel protein type 4 subunit alpha, polymer, 35 residues, 4033.070 Da.

Natural source: Common Name: Rat Taxonomy ID: 10116 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Rattus not available

Experimental source: Production method: chemical synthesis

Entity Sequences (FASTA):
Sodium channel protein type 4 subunit alpha: KKKGICFFCSYIIISFLIVV NMYIAIILENFKKKX

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	111

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	entity_1	1

Entities:

Entity 1, entity_1 35 residues - 4033.070 Da.

1

LYS

GLY

ILE

CYS

PHE

CYS

SER

2

TYR

ILE

SER

PHE

LEU

ILE

VAL

3

ASN

MET

TYR

ILE

ALA

ILE

LEU

GLU

ASN

4

PHE

LYS

NH2

Samples:

sample_1: DMPC, d-54, 69%; peptide, U-13C I23, L17, V20, 16%

sample_2: DMPC, d-54, 69%; peptide, U-13C G, I, F, L, V, A, 16%

sample_conditions_1: ionic strength: 0 mM; pH: 7.4; pressure: 1 atm; temperature: 213 K

Experiments:

Name	Sample	Sample state	Sample conditions
13C-13C dipolar-assisted rotational resonance	sample_1	anisotropic	sample_conditions_1
13C-13C dipolar-assisted rotational resonance	sample_2	anisotropic	sample_conditions_1
13C observation of 2H-selective 1H-depolarization	sample_1	anisotropic	sample_conditions_1

Software:

CHARMM, M. KARPLUS ET AL, HARVARD UNIVERSITY - refinement

DELTA - structure solution

FELIX - structure solution

SPARKY - structure solution

NMR spectrometers:

Varian Infinity Plus 700 700 MHz